The effects of different lighting schedules in shift work and the chronic mild stress on the behaviour of Wistar rats
Agnes
Müller1*,
Rita
Józsa2,
Nikolett
Gál1,
József
Betlehem1,
János
Sándor1,
Norbert
Csókás1,
Gábor
Nagy1,
Imre
Boncz1,
Dóra
Reglodi2,
Ildikó
Kriszbacher1,
Franz
Halberg1,
Germanie
Cornélissen1 and
András
Oláh1
-
1
University of Pécs, Nursing Sciences Department, Faculty of Health Sciences, Hungary
-
2
University of Pécs, Department of Anatomy, Medical School, Hungary
Aim: The aim of the present study is to model the different types of shift work schedules in an animal experimental model in order to examine the effects of lighting shifts and the chronic mild stress on anxiety-like behaviour. Material and method: Adult male and female Wistar rats of same age (n=61) were used. The animals were divided into four groups of 15-16 animals each. The internationally most frequently used night shift schedules were applied in three groups of animals (4DL/3LD; 8DL/6LD; 2DL/2LD) beside a group of animals kept in a normal LD cycle (12 hour light-L/12 hour dark-D). In addition, in all lighting regimen the groups were further divided in two groups and one of them were subjected to chronic mild stress. Degree of anxiety was evaluated in light-dark box. Multiple one-way analyses of variance (ANOVAs) were used to examine the differences between the groups according to the variances (sex, lighting regimens and chronic mild stress) and time spent in the light compartment, the number of changing the compartments, and the latency of stepping through from the dark to the light section. Results: Regarding the examined 3 variables in groups kept under different lighting schedules compared to those kept under normal LD lighting regimen (time spent in light: 107,0s; changing the compartments of the box: 9 times; latency: 4,8s), significant differences were found: animals kept in the 4DL/3 LD and 2DL/2LD schedules spent less time in the light compartment, indicating decreased exploratory behaviour and had increase in latency (the latency time for the first passage from the light compartment to the dark one), indicating anxiety-like behaviour (4DL/3 LD group ― time spent in light: 65,72s, p=0,005; changing the compartments: 7,2 times, p=0,079 /nearly significant/; latency: 10,65s, p=0,011 and 2DL/2LD group ― time spent in light: 74,72s, p=0,025; changing the compartments: 5,47 times, p=0,001; latency: 9,33s, p=0,045), while no statistically significant differences were found in the 8DL/6LD group in any of the variables (time spent in light: 84,53s, p=0,113; changing the compartments: 7,4 times, p=0,118; latency: 6,5s, p=0,45). Conclusion: According to the presented results it appears that phase shifts different from the normal light/dark regimen leads to anxiety also without chronic mild stress. The results observed in group 8DL/6LD show the closest approximations of parameters observed in the LD group, suggesting that this schedule applied in shift work is less harmful to health, and provides the best way of regeneration.
Keywords: shift work, chronic mild stress (CMS), light-dark box.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Cognition and behavior
Citation:
Müller
A,
Józsa
R,
Gál
N,
Betlehem
J,
Sándor
J,
Csókás
N,
Nagy
G,
Boncz
I,
Reglodi
D,
Kriszbacher
I,
Halberg
F,
Cornélissen
G and
Oláh
A
(2010). The effects of different lighting schedules in shift work and the chronic mild stress on the behaviour of Wistar rats.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00170
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Received:
30 Apr 2010;
Published Online:
30 Apr 2010.
*
Correspondence:
Agnes Müller, University of Pécs, Nursing Sciences Department, Faculty of Health Sciences, Pécs, Hungary, agnes.muller@etk.pte.hu