The proteomic effects of sleep deprivation
Attila
Simor1*,
Péter
Gulyássy1,
Katalin
Völgyi1,
Charlotte
Markussen2,
Eva
Hunyadi-Gulyás3,
Zsuzsanna
Darula4,
Katalin
Medzihradszky4,
András
Czurkó1, 4,
Gábor
Juhász1 and
Katalin
A.
Kékesi1, 5
-
1
Eötvös Loránd University, Institute of Biology, Laboratory of Proteomics, Hungary
-
2
University of Bergen, Department of Physiology, Norway
-
3
University of Szeged, Department of Medical Chemistry, Hungary
-
4
Hungarian Academy of Sciences, Proteomics Research Group, Biological Research Center, Hungary
-
5
Eötvös Loránd University, Department of Neurobiology and Physiology, Hungary
Sleep has function in restoration of brain functions in waking and in memory consolidation, however sleep deprivation induces sensory impairment, hallucinations and sever somatic and sensory symptoms. Because memory consolidation requires de novo protein synthesis and also restorative processes in brain cells are based on protein synthesis and degradation, understanding how the brain proteome changes under sleep deprivation is an important issue. In the present study we made 8 hours sleep deprivation by gentle handling to reduce stress response as much as possible. 12 hours after finishing the sleep deprivation we sacrificed the rats and measured the changes in the thalamic and cortical proteome of rats by 2D-DIGE. We obtained a significant difference in number of proteins changed in the cortex (93) and in the thalamus (11) indicating that the cortex involved in cognition, learning and sensory functions which is a subject of an extensive proteome change. Interestingly the thalamus which is a relay structure between the sensory organs and the cortex did not respond to sleep deprivation as extensively as the cortex did. Protein interaction modelling of the data shows the major functional processes changing in sleep deprivation.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Cellular neuroscience
Citation:
Simor
A,
Gulyássy
P,
Völgyi
K,
Markussen
C,
Hunyadi-Gulyás
E,
Darula
Z,
Medzihradszky
K,
Czurkó
A,
Juhász
G and
Kékesi
KA
(2010). The proteomic effects of sleep deprivation.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00179
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Received:
30 Apr 2010;
Published Online:
30 Apr 2010.
*
Correspondence:
Attila Simor, Eötvös Loránd University, Institute of Biology, Laboratory of Proteomics, Budapest, Hungary, plaut_vincent@freemail.hu