Kainate, AMPA and NMDA receptor plasticity in animal models of epilepsy
-
1
Faculty of Medicine, University of Szeged, Department of Anatomy, Hungary
We investigated the reorganization of ionotropic glutamate receptor subunits in the cerebral cortex of adult mice and rats suffering repeated seizures, with histoblotting method (Tonnes et al, 1999). Kainate subunit GluK5, AMPA subunits GluA1 and GluA2 and NMDA subunit GluN1 were detected using coronal plane hippocampal sections, and evaluated with optical densitometry. Experiment 1: animals were subjected to daily, brief seizures elicited with systemic 4-aminopyridine, for 12 days. This treatment did not cause neuronal loss (Vizi et al, 2004). GluK5 decreased significantly in stratum lucidum of CA3, GluA1 increased in CA1 and CA3, GluA2 decreased in CA1 and dentate gyrus, significantly. GluN1 did not change. Our results indicate the rearrangement of receptor subunits: as a consequence, the calcium permeability of AMPA receptors increased significantly (Borbély et al, 2009). Experiment 2: animals were subjected to pilocarpine-induced status epilepticus (SE). Fifty days following the SE, animals were sacrificed and histoblotting performed. GluK5 and GluN1 were evaluated with optical densitometry, axonal sprouting with Timm-staining and enkephalin immunohistochemistry. Cell number was counted on Neu-N-immunostained sections. GluK5 and GluN1 decreased significantly in the dentate gyrus and in CA3. The number of granule cells and hilar neurons decreased, too. Strong axonal sprouting was detected in CA3 and in the supragranular section of the molecular layer of the dentate gyrus. The results indicate significant decrease of the density of kainate and NMDA receptors in the CA3 and dentate gyrus.
Borbély S, Dobó E, Czégé D, Molnár E, Bakos M, Szűcs B, Vincze A, Világi I, Mihály A, Neuroscience 159, 358-368 (2009).
Tonnes J, Stierli B, Cerletti C, Behrmann JT, Molnar E, Streit P, J Neurochemistry 73, 2195-2205 (1999).
Vizi S, Bagosi A, Krisztin-Péva B, Gulya K, Mihály A, Mol Brain Research 131, 110-118 (2004).
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Oral Presentation
Topic:
Kainate receptors in synaptic plasticity
Citation:
Mihály
A
(2010). Kainate, AMPA and NMDA receptor plasticity in animal models of epilepsy.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00208
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
04 May 2010;
Published Online:
04 May 2010.
*
Correspondence:
András Mihály, Faculty of Medicine, University of Szeged, Department of Anatomy, Szeged, Hungary, mihaly@anatomy.szote.u-szeged.hu