Dual-Superfusion Slice Chamber
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1
Supertech International Ltd., Hungary
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2
Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary
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3
Exasol Ltd., Hungary
Dual-superfusion slice chamber: a novel apparatus to obtain network oscillations and network driven activities during visually-guided electrophysiological recordings and optical imaging.
It is a unique, worldwide patented idea. This is a brand new type of slice chamber for electrophysiological recordings and/or in vitro imaging developed by Norbert Hajos, Ph.D. The dual-superfusion slice chamber is recommended for all types of slice recordings, but it is indispensable for those investigations, where oxygen is a limiting factor of neuronal function, e.g. network oscillations (Hajos et al., European Journal of Neuroscience, Vol. 29, pp. 319-327, 2009).
Dual-superfusion of submerged slices increases the vitality of cells, since e.g. oxygen as well as other materials from both sides can freely and more effectively diffuse into the thick slices. In the most widely used slice chambers, where the slices are sitting on a glass coverslip, the oxygenation and the drug diffusion is achieved only from one side.
We invented a chamber insert where slices are sitting on a mesh and the shape of the insert supports laminar flow around the submerged slices ensuring the better oxygenation of slices. The mesh divides the chamber into two parts. Both the upper (above the mesh), and the lower (below the mesh) parts of the chamber have got 2 mm of height. By using this type of chamber design, the flow rate of the superfused solutions could be kept as low as 3-5 ml/min, yet the oxygenation of the tissue could be maximally maintained. In the case, when experiments are desired to perform at higher than room temperature, then not only the flow rate but the temperature, as well, should be maintained as equivalent as possible below and above the slices. To achieve this, a Dual-channel In-line Solution Heater is recommended to use, where the temperature of two inlets is controlled in a single heater head to ensure the same temperature.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Abstracts
Citation:
Niedetzky
C,
Rózsa
B,
Puskás
Z and
Hájos
N
(2010). Dual-Superfusion Slice Chamber.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00254
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Received:
05 May 2010;
Published Online:
05 May 2010.
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Correspondence:
Csaba Niedetzky, Supertech International Ltd., Pécs, Hungary, csaba.niedetzky@super-tech.eu