Construction of database of the 3-dimentional digital brain atlas of a rodent Degu (Octodon degu)
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1
BSI, RIKEN, Lab for Symbol Cogn Develop, Japan
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2
BSI, RIKEN, Neuroinformatics Japan Center (NIJC), Japan
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3
BSI, RIKEN, Support Unit for Neuromorphol Analysis, Japan
Purpose: The Caviomorph rodent degu (Octodon degu) is native to highland of Chile. Their social systems are unique and quite different from those of rats and mice. The degus live in tight-knit, extended family unit with complicated social behaviors, such as vocal communications. They are diurnal, so that they are well-sighted. We demonstrated that Degu can be trained to use a tool, T-shaped rake. Thus, degu has recently become increasingly important as experimental animals in researches of higher cognitive brain function. There exists only a line-drawing brain atlas of degu, but not histological or MRI brain atlases. So, we constructed stereotaxic histological and MRI brain atlases of degu to make it easier to find their anatomical structures by constructing 3-dimentional model with various functions such as the rotating and sectioning functions. Methods: To confirm the stereotaxic orientation of sections in the brain used for the atlas, reference tracts were made perpendicular to the horizontal (1mm anterior and 2mm right lateral from ear bar zero) and coronal (4mm upper and 1.5mm right lateral from ear bar zero). The animal's head was removed and stored for a minimum of 2 days in 4% Paraformaldehyde (PFA). After fixing the brain, the MRI images are acquired by Bruker 400 Ultrashield (9.4T). The frontal and parietal bones were removed to expose the brain. Gelatin-embedded brain was coronally cut on a freezing microtome at 50µ of thickness. A series of Nissl sections were prepared for microscopic imaging. The photographs of stained brain sections were taken with the Olympus virtual slide system (VS-100). After trimming of brain pictures using Adobe Photoshop, we have constructed 3-dimentional digital volume-rendering brain model of degu by a software "SG-eye" which we originally developed with company "Fiatlux". We have annotated the brain regions according to the anatomical terms in "The Rat Brain in Stereotaxic Coordinates" by Paxinos, G. and Watson, C. We have constructed database of stereotaxic 3-dimensional brain atlas of degu with the Neuroinformatics Base Platform System "XooNIps" which have developed by Neuroinfomatic Japan center (NIJC) of Brain Science Institute (BSI). This project is supported by BSI-NIJC. Results: We have constructed database of stereotaxic 3-dimensional brain atlas of degu with Xoonips. In the 3-dimentional model, we are able to see histological images and MRI images at the same cross sections of the 3-dimentional model in the same screen. The advantages of this brain atlas are axis readjusting, freely rotating and sectioning function. These functions help us to see the model from any angels we want, and make 3-dimensional brain atlas of degu more useful. Conclusions: Newly developed 3-dimensional stereotaxic histological brain atlas of degu will be very useful for researchers working in various fields of neuroscience, such as electrophysiological recordings, lesion studies, tract tracing, drug administration experiments, behavioral examination, and so on.
Conference:
Neuroinformatics 2010 , Kobe, Japan, 30 Aug - 1 Sep, 2010.
Presentation Type:
Oral Presentation
Topic:
Digital atlasing
Citation:
Kumazawa
N,
Katayama
M,
Usui
S,
Hashikawa
T and
Iriki
A
(2010). Construction of database of the 3-dimentional digital brain atlas of a rodent Degu (Octodon degu).
Front. Neurosci.
Conference Abstract:
Neuroinformatics 2010 .
doi: 10.3389/conf.fnins.2010.13.00029
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Received:
10 Jun 2010;
Published Online:
10 Jun 2010.
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Correspondence:
Noriko Kumazawa, BSI, RIKEN, Lab for Symbol Cogn Develop, Saitama, Japan, nkuma@brain.riken.jp