Gender-linked differences in rat brain after transient global cerebral ischemia
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1
University of Szeged, Department of Physiology, Hungary
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2
University of Belgrade, “Vinča” Institute of Nuclear Sciences, Serbia
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3
University of Szeged, Department of Medical Physics & Informatics, Hungary
The aim of our study was to examine putative gender differences in expression of various proteins that have a specific role in progression of brain injury and cell repair following transient global ischemia (TGI). Adult male (♂) and ovariectomized female (♀) rats (n=42) were divided into 3 main groups: naive, sham-operated and TGI. TGI was induced by occlusion of the common carotid arteries with hypovolemic hypotension (RR~40 mmHg; for 5 or 10 min [TGI5; TGI10]). Three days after TGI animals were sacrificed. Western blot was used to detect connexin, pro- and antioxidant enzymes as well as pro- and antiapoptotic protein expression levels in hippocampus. TGI5 in ♂ rats caused a decrease in nNOS expression (81,0±6,3% of control) that could be due to neuronal loss; while the observed decrease in Cx30 (82,7±2,3% of control) could prevent transduction of apoptotic signaling. However, in these animals, the expression of MnSOD, Bax, and Bcl-2 was less affected. TGI10 resulted in death of all ♂ animals within a day. In ♀ rats, TGI5 did not significantly change the expression of investigated proteins, but after TGI10, Bcl-2/Bax protein ratio was enhanced, which could initiate a cascade of events leading to neuroprotection. This study shows that ♂ rats are more sensitive to TGI insult than ♀ rats. Further, TGI affects protein expression differently in ♂ and ♀ animals which might explain observed gender differences in sensitivity to brain ischemia.
Acknowledgements
OTKA K81266, ETT 375-04 & TAMOP 4.2.1/B
Keywords:
disorders,
Neuroscience
Conference:
13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011.
Presentation Type:
Abstract
Topic:
Disorders
Citation:
Hugyecz
M,
Drakulic
D,
Farkas
E,
Hertelendy
P,
Tóth
J and
Bari
F
(2011). Gender-linked differences in rat brain after transient global cerebral ischemia.
Front. Neurosci.
Conference Abstract:
13th Conference of the Hungarian Neuroscience Society (MITT).
doi: 10.3389/conf.fnins.2011.84.00142
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Received:
03 Mar 2011;
Published Online:
23 Mar 2011.
*
Correspondence:
Dr. M. Hugyecz, University of Szeged, Department of Physiology, Szeged, Hungary, hugyeczmarietta@gmail.com