Unraveling the role of Cdk1 in postnatal neurogenesis
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1
GIGA Neurosciences-University of Liege, Belgium
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2
Affichem, France
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3
Institute of Molecular and Cell Biology, Singapore
Age-related neurological disorders, including stroke and neurodegenerative diseases are becoming a major health care problem in many countries as average life expectancy is increasing. One appealing therapeutic strategy to treat neurological disorders would consist of recruiting endogenous neural precursor cells (NPCs) to replace the lost neurons. NPCs are present in two specific areas of the postnatal brain, the dentate gyrus (DG) of the hippocampus and the subventricular zone (SVZ) of the lateral ventricles. These NPCs give rise to neurons throughout life, a phenomenon termed postnatal neurogenesis. A prerequisite for the development of new therapeutic strategies involving postnatal NPCs is a better understanding of their molecular regulation. In this context, both extrinsic factors and intrinsic factors have been identified. For instance, the family of cell cycle regulators cyclin-dependent kinases (Cdks) are key regulators of postnatal neurogenesis since genetic invalidation of interphase Cdk2 or Cdk6 leads to a decrease of NPCs proliferation. Here, we investigated the role of Cdk1, an essential Cdk for M-phase in several tissues, in postnatal neurogenesis. Towards that purpose, we crossed mice bearing a conditional Cdk1 allele (Cdk1lox) with mice expressing a tamoxifen-inducible form of the Cre-recombinase under the control of the Sox2 promoter (Sox2CreER), allowing us to specifically delete Cdk1 in Sox2+ NPCs in the postnatal brain. Following Cdk1 loss, our preliminary results show a decrease in the percentage of proliferating Sox2+ cells as well as a decrease in the number of phospho-histone H3+ (ph-h3) in the DG, but there is no difference in the number of cells positive for the apoptotic marker activated-caspase 3. Interestingly, we also observed a long-term depletion of the pool of Sox2+ in the absence of Cdk1. Altogether, these data suggest a crucial role for Cdk1 in postnatal neurogenesis, but further investigations are required to identify its precise requirement in this process.
Keywords:
adult neurogenesis,
Cell Cycle,
Cdk1,
Neural Stem Cells,
proliferation
Conference:
11th National Congress of the Belgian Society for Neuroscience, Mons, Belgium, 22 May - 22 May, 2015.
Presentation Type:
Poster presentation
Topic:
Neuroscience
Citation:
Marlier
Q,
Verteneuil
S,
Vandenbosch
R,
Caron
N,
Kaldis
P,
Nguyen
L and
Malgrange
B
(2015). Unraveling the role of Cdk1 in postnatal neurogenesis.
Front. Neurosci.
Conference Abstract:
11th National Congress of the Belgian Society for Neuroscience.
doi: 10.3389/conf.fnins.2015.89.00069
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Received:
30 Apr 2015;
Published Online:
05 May 2015.
*
Correspondence:
Mr. Quentin Marlier, GIGA Neurosciences-University of Liege, Liège, 4000, Belgium, quentin.marlier@ulg.ac.be
Mr. Sebastien Verteneuil, GIGA Neurosciences-University of Liege, Liège, 4000, Belgium, sebastien.verteneuil@hotmail.fr