Extracellular recordings of neuronal activity by protruding microelectrodes array
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1
University of Antwerp, Dept. Biomedical Sciences, Belgium
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2
Natural and Medical Sciences Institute, Germany
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3
Institute of Life Science, Hebrew University, Israel
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4
University of Antwerp, Dept. Veterinary Sciences, Belgium
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5
Neuro-Electronic Research in Flanders, Belgium
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6
University of Sheffield, Dept. Computer Science, United Kingdom
Multi-electrode arrays (MEAs) are widely used for the investigation of neuronal networks properties and drug screening. The main advantage of this method is a simultaneous registration of electrical activity from a large number of neurons during long periods (days or weeks). However, a major disadvantage of MEA recordings relates to the low signal to noise ratio resulting in failure of detecting subthreshold synaptic events and thus not capturing an essential part of information. In view of the development of novel MEAs with improved recording quality, we investigated the properties and validity of mushroom shape microelectrodes (gold μE).
Using dissociated neuronal cultures, the present study revealedthat neuronal cells are able to engulf gold μEs and that the presence of protruding gold μEs do not impact on adhesion and viability of these neurons. In addition, we could show that µEs do not alter passive/active membrane properties of neurons or their network properties. Furthermore, this type of microelectrodes in combination with a conducting polymer coating significantly improves the quality of extracellular recordings. We can conclude that µEs offer further perspectives in enhancing the quality and sensitivity of MEAs and thus might provide an added value in our future understanding of neuronal network behaviour.
Acknowledgements
J. Couto for discussions; D. Van Dyck for the excellent technical assistance. Financial support from
the European Commission (Collaborative Research Project “BRAINLEAP”), from the Research
Foundation Flanders (FWO, grant n. G.0888.12N), and from the Belgian Science Policy Office
(BELSPO)
Keywords:
MEA,
protruding electrode,
action potential,
Engulfment,
multielectrode recordings
Conference:
11th National Congress of the Belgian Society for Neuroscience, Mons, Belgium, 22 May - 22 May, 2015.
Presentation Type:
Poster presentation
Topic:
Neuroscience
Citation:
Moskalyuk
A,
Samba
R,
Spira
ME,
Pintelon
I,
Timmermans
J and
Giugliano
M
(2015). Extracellular recordings of neuronal activity by protruding microelectrodes array.
Front. Neurosci.
Conference Abstract:
11th National Congress of the Belgian Society for Neuroscience.
doi: 10.3389/conf.fnins.2015.89.00072
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Received:
30 Apr 2015;
Published Online:
05 May 2015.
*
Correspondence:
Prof. Michele Giugliano, University of Antwerp, Dept. Biomedical Sciences, Antwerp, 2610, Belgium, michele.giugliano@uantwerpen.be