Current Source Density Estimation for Single Neurons
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1
Wigner Research Center for Physics of the Hungarian Academy of Sciences, Department of Theory, Hungary
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2
Budapest University of Technology and Economics, Department of Measurement and Information Systems, Faculty of Electrical Engineering and Informatics, Hungary
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3
Nencki Institute of Experimental Biology Polish Academy of Sciences, Department of Neurophysiology, Poland
Recent developments of multielectrode technology made it possible to measure the extracellular potential generated in the neural tissue with spatial precision on the order of tens of micrometers and on submillisecond time scale. Combining such measurements with imaging of single neurons within the studied tissue opens up new experimental possibilities for estimating distribution of current sources along a dendritic tree.
In this work we show that if we are able to relate part of the recording of extracellular potential to a specific cell of known morphology we can estimate the spatiotemporal distribution of transmembrane currents along it. We present here an extension of the kernel CSD method (Potworowski et al., 2012) applicable in such case. We test it on several model neurons of progressively complicated morphologies from ball-and-stick to realistic, up to analysis of simulated neuron activity embedded in a substantial working network (Traub et al, 2005). We discuss the caveats and possibilities of this new approach.
Acknowledgements
Network simulations were in part performed in the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw, under grants G35-7 and POWIEW. Research supported by the grants from the Polish Ministry of Regional Development, POIG.02.03.00-00-003/09, POIG.02.03.00-00-018/08, and from the Polish Ministry of Science and Higher Education: 5428/B/P01/2010/39 and IP2011~030971.
References
Potworowski J, Jakuczun W, Leski S, Wójcik DK (2012) Kernel current source density method. Neural Comput 24: 541□575.
Traub RD, Contreras D, Cunningham MO, Murray H, LeBeau FEN, et al. (2005) Single-column thalamocortical network model exhibiting gamma oscillations, sleep spindles, and epileptogenic bursts. J Neurophysiol 93: 2194□2232.
Keywords:
CSD (current-source density),
LFP analysis,
morphology,
kernel CSD method,
imaging techniques
Conference:
4th NAMASEN Training Workshop - Dendrites 2014, Heraklion, Greece, 1 Jul - 4 Jul, 2014.
Presentation Type:
Poster presentation
Topic:
morphological and functional characterizations
Citation:
Cserpán
D,
Głąbska
H,
Somogyvári
Z and
Wójcik
DK
(2014). Current Source Density Estimation for Single Neurons.
Front. Syst. Neurosci.
Conference Abstract:
4th NAMASEN Training Workshop - Dendrites 2014.
doi: 10.3389/conf.fnsys.2014.05.00031
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Received:
11 Apr 2014;
Published Online:
12 Jun 2014.
*
Correspondence:
Miss. Dorottya Cserpán, Wigner Research Center for Physics of the Hungarian Academy of Sciences, Department of Theory, Budapest, H-1121, Hungary, cserpan@mit.bme.hu