Endosulfan-induced lipid oxidation in Wistar rats: the protective action of vitamins C and E
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1
Aristotle University of Thessaloniki, Laboratory of Forensic Medicine and Toxicology, Greece
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2
Aristotle University of Thessaloniki, Department of Food Hygiene and Technology, Greece
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3
Aristotle University of Thessaloniki, Lab. of Animal Production Economics, Greece
Introduction. Endosulfan, an organochlorine compound used as an insecticide, has found worldwide application and its residues have often entered the food chain and constituted a health hazard. The effect of endosulfan oral administration to Wistar rats, with respect to lipid oxidation and the protective effect of vitamins C and E, was studied.
Methods. Two levels of endosulfan (1.5 and 10 mg/kg of rat bodyweight) and vitamins C and E were administered at 500 mg/kg b.w. (in water) and 200 mg/kg of feed, respectively for 6 weeks. A total of 144 Wistar rats were equally divided into 3 treatment groups (control, +endosulfan 1.5, +endosulfan 10), for each sex. Each group was divided in 4 subgroups (no vitamins added, +vitamin C, +vitamin E, +vitamin C and E) of 6 rats. Heart, liver, kidney, testes and brain samples were analyzed for Malondialdehyde (MDA) with a third-order derivative spectrophotometric method. One-way ANOVA and Duncan test or Kruskal–Wallis and Mann-Whitney npar tests (in case of non-normality) were applied for statistical evaluation of MDA concentrations.
Results. Evaluation of data showed a decrease in MDA levels in all tissues examined, when vitamin C or vitamin E or their combination was administered, in both sexes (P≤0.05). In male rats, the combination of vitamins C and E resulted in significant decrease of MDA levels in kidney, liver and heart for all treatments, while in testes only for endosulfan 1.5 and in brain for endosulfan 1.5 and 10 groups. Vitamin E administration resulted in lower levels of MDA in liver and heart for all treatments, while in testes only for endosulfan 1.5 and in brain for endosulfan 1.5 and 10 groups (P≤0.05). Vitamin C administration resulted in significantly lower levels of MDA in testes and brain for endosulfan 1.5 groups, while in liver for all treatments. In female rats, the combination of vitamins C and E resulted in significant decrease of MDA levels in liver, brain and heart for all treatments, while in kidney only for control and endosulfan 10 groups. Vitamin E administration resulted in significantly lower levels of MDA in liver for all treatments, while in heart and brain only for endosulfan groups. Vitamin C administration resulted in lower levels of MDA in heart and liver for endosulfan 1.5, while in brain for endosulfan 10 groups (P≤0.05).
Conclusions. The results of the present study indicate that vitamins C and E (alone or in combination) offer protection against lipid oxidation, induced by endosulfan.
Keywords:
Endosulfan,
lipid oxidation,
vitamin C,
Vitamin E
Conference:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010, Thessaloniki, Greece, 1 Oct - 5 Oct, 2010.
Presentation Type:
Poster
Topic:
Xenobiotic toxicity
Citation:
Eleftheriadou
E,
Papapanagiotou
E,
Fletouris
D,
Batzios
C,
Tsoukali
H and
Njau
SN
(2010). Endosulfan-induced lipid oxidation in Wistar rats: the protective action of vitamins C and E.
Front. Pharmacol.
Conference Abstract:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010.
doi: 10.3389/conf.fphar.2010.60.00119
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Received:
28 Oct 2010;
Published Online:
04 Nov 2010.
*
Correspondence:
Dr. Dimitrios Fletouris, Aristotle University of Thessaloniki, Department of Food Hygiene and Technology, Thessaloniki, 54124, Greece, djflet@vet.auth.gr