Effects of ursodeoxycholic acids on oxidative stress level in hepatic tissue of rats with extrahepatic cholestasis
-
1
University of Nis, Institute of Biochemistry, Serbia
Introduction. Accumulation of toxic hydrophobic bile acids (BA) during cholestasis can activate Fas death receptors and increase production of reactive oxygen species (ROS) simultaneously inducing apoptosis of
hepatocytes. The major beneficial effects of treatment with ursodeoxycholic acid (UDCA) are protection against cytotoxicity of BA, stimulation of hepatobiliary secretion, antioxidative activity and blocking
the apoptotic signal.The aim of the present study was to evaluate the effect of UDCA on oxidative stress in liver tissue of bile duct-ligated (BDL) rats.
Methods. Four groups of Wistar rats were investigated: I-control (sham operated), II-rats treated with UDCA (25mg/kg b.w. i.g.), III-rats with common bile duct-ligation (BDL), IV-cholestatic rats treated with UDCA. The animals were killed after 9 days.
Results. Increased plasma activity of GGT, AF (cholestatic markers) and concentration of BA in BDL rats was registered, while oral administration of UDCA induce decreasing of activity and concentration of all mentioned parameters (p<0.001). Lipid peroxidation-MDA and oxidative modification of proteins-carbonyl group content are significantly increased in BDL group (p<0.05). UDCA was found to be protective in liver of BDL rats. It decreases MDA levels (p<0.01); moderately decrease carbonyl group, compared with cholestatic rats.
Conclusions. These results support a novel effects of UDCA in reducing oxidative damage and maintaining of antioxidant defenses in cholestatic liver.
Keywords:
usodeoxycholic acids,
Oxidative Stress,
extrahepatic cholestasis,
Rats
Conference:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010, Thessaloniki, Greece, 1 Oct - 5 Oct, 2010.
Presentation Type:
Poster
Topic:
Xenobiotic toxicity
Citation:
Jovic
M,
Jovic
M and
Sokolovic
D
(2010). Effects of ursodeoxycholic acids on oxidative stress level in hepatic tissue of rats with extrahepatic cholestasis.
Front. Pharmacol.
Conference Abstract:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010.
doi: 10.3389/conf.fphar.2010.60.00132
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
28 Oct 2010;
Published Online:
04 Nov 2010.
*
Correspondence:
Dr. Marko Jovic, University of Nis, Institute of Biochemistry, Nis, Serbia, markojovic10@yahoo.com