Assessment of Antioxidant Activity of various compounds used in sport by a Combination of Molecular Methods
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1
University of Thessaly, Department of Biochemistry and Biotechnology, Greece
Physical activity increases the production of reactive oxygen species (ROS), which are important biological molecules that may damage a number of cell constituents. Several studies have shown that fruits, vegetables and common beverages as well as herbs and plants are rich sources of antioxidant compounds and are used extensively in sport. In the present report, a battery of in vitro methods for the identification of antioxidant agents used in athletism are presented. These methods include: i) the bleomycin-induced mutations in Salmonella typhimurium TA102 cells, ii) the H2O2-induced sister chromatid exchanges (SCEs) in human peripheral blood lymphocytes, iii) the OH. or peroxyl-radical induced DNA breakage and iv) free radical scavenging activity. These methods are used for the identification of agents which prevent reactive oxygen species (ROS) mediated tissue damage and are useful in the evaluation of various compounds the antioxidant activity of which in sport is controversial.
Keywords:
mitomycin c,
DNA breakage,
Salmonella typhimurium,
Bleomycin,
topoisomerase I,
Oxidative Stress,
ROS,
plant phenolics,
antioxidant,
Sport
Conference:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010, Thessaloniki, Greece, 1 Oct - 5 Oct, 2010.
Presentation Type:
Oral
Topic:
Xenobiotic toxicity
Citation:
Kouretas
D
(2010). Assessment of Antioxidant Activity of various compounds used in sport by a Combination of Molecular Methods.
Front. Pharmacol.
Conference Abstract:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010.
doi: 10.3389/conf.fphar.2010.60.00148
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Received:
28 Oct 2010;
Published Online:
04 Nov 2010.
*
Correspondence:
Dr. Demetrios Kouretas, University of Thessaly, Department of Biochemistry and Biotechnology, Larissa, GR-41221, Greece, dkouret@uth.gr