Immunohistochemical study of androgen receptors in rat uterus after administration of testosterone
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1
Aristotle University of Thessaloniki, Department of Pharmacology, Greece
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2
Aristotle University of Thessaloniki, Laboratory of Histology-Embryology and Anthropology, Greece
Aim of our study was to confirm published data on androgen receptors (ARs) localized in uterus, to examine the induction of ARs in the uteri of female Wistar rats when administered with high dosages of testosterone, to measure any effects on weight and to record any toxic effects on the endometrial structure or function. Female adult Wistar rats (6 weeks old, weighing from 150 to 200 gr, fed ad libitum), undergoing the same estrus cycle phase, were divided into three groups, according to the administration dose of testosterone. In the first group, 25 mg of testosterone were administered intramuscularly, while the half dose (of 12.5 mg) was administered to the second group. The third group was the control group. Ten days after the testosterone administration, euthanasia via ether was performed to the rats, while tissue samples from the uterus mucosa were obtained, fixed and prepared for immunohistochemical study by polymerization. In the first group, of high testosterone dose, positive expression of AR was observed in many hepatic cells of the uterus mucosa and also in adenocytes of the basic mucosa’s layer. In the second group, which took lower testosterone dose, the expression of AR in mucosa’s epithelium was less positive to negative in some of them. Uterus weight of rats did not differ significantly among groups, with the high testosterone dosage group to present slightly lower values (median weight of 0.404 gr) than controls (median weight of 0.461 gr), while uteri of rats treated with high testosterone dosages were presented macroscopically with mild hyperemia and hypertrophy. In agreement with bibliographical data, high doses of androgens give positive expression of ARs in the uterus’ mucosa epithelial cells regarding either their basal layer or their functional layer.
Keywords:
Testosterone,
rat uterus,
androgen receptor
Conference:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010, Thessaloniki, Greece, 1 Oct - 5 Oct, 2010.
Presentation Type:
Poster
Topic:
Xenobiotics and reproduction
Citation:
Kyriakidis
I,
Dermentzopoulou-Theodoridou
M,
Papamitsou
T,
Makaronidis
I,
Tsanakalis
F,
Manthos
A and
Papaioannidou
P
(2010). Immunohistochemical study of androgen receptors in rat uterus after administration of testosterone.
Front. Pharmacol.
Conference Abstract:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010.
doi: 10.3389/conf.fphar.2010.60.00152
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Received:
05 Mar 2011;
Published Online:
04 Nov 2010.
*
Correspondence:
Dr. Ioannis Kyriakidis, Aristotle University of Thessaloniki, Department of Pharmacology, Thessaloniki, Greece, kyriakidis@med.uoc.gr