Ultrastructural changes in rat oocytes after treatment with testosterone enanthate
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1
Aristotle University of Thessaloniki, Department of Pharmacology, Greece
Aim of this work was to study the changes in the ultrastructure of oocytes taken from ovaries of healthy adult female rats after treatment with high testosterone doses. Wistar female rats (6 weeks old, weighing from 165 to 180 gr, fed ad libitum) were used. In the study group, rats were administered with one single dose of 25 mg of testosterone enanthate intramuscularly. In the second group the animals received normal saline intramuscularly. Ten days after treatment euthanasia was performed to the rats, while a small portion of ovarian tissue was taken and fixed in glutaraldehyde 3 %. Ovaries in testosterone treated rats were found with a median weight of 0.126 gr –slightly higher than controls- and were presented macroscopically with hyperemia and hypertrophy, while all rats were found to undergo the beginning of their estrus cycle (i.e. two cycles after the injection). After the appropriate preparation, ovarian tissue was examined under a transmission electron microscope. In the study group, perinuclear and intracellular edema was observed; several mitochondria presented decreased crest number and edematic appearance. In various places, the cisternae of the granular endoplasmic reticulum (GER) were increased and myelinoid bodies appeared. It seems that testosterone changes the normal ultrastructure of oocytes, and this may have an impact on their physiological functions.
Keywords:
oocyte,
Testosterone,
rat,
ultrastructure
Conference:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010, Thessaloniki, Greece, 1 Oct - 5 Oct, 2010.
Presentation Type:
Poster
Topic:
Xenobiotics and reproduction
Citation:
Makaronidis
I,
Kyriakidis
I,
Tsanakalis
F and
Kotoula
M
(2010). Ultrastructural changes in rat oocytes after treatment with testosterone enanthate.
Front. Pharmacol.
Conference Abstract:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010.
doi: 10.3389/conf.fphar.2010.60.00158
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Received:
05 Mar 2011;
Published Online:
04 Nov 2010.
*
Correspondence:
Dr. Ioannis Kyriakidis, Aristotle University of Thessaloniki, Department of Pharmacology, Thessaloniki, Greece, kyriakidis@med.uoc.gr