The impact of cholesterol rich diet upon old Wistar rat brain metabolism
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1
University of Medicine and Pharmacy”Carol Davila”, Romania
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2
National Institute of gerontology and Geriatrics”Ana Aslan”, Romania
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3
Victor babes Institute, Romania
Introduction. The brain and endocrine glands are common sites for fat soluble toxins to accumulate due to decreasing liver capacity of detoxification during aging and this may result in brain dysfunction and hormone imbalance.
Oxidative damage via nitric oxide has been implicated in Parkinson’s disease.
Glucuronidation represents a major metabolic protection of brain against chemical aggressions at blood barrier interfaces and plays an important role in the maintanace of cellular integrity and homeostasis during postnatal development and brain aging.
Glucuronidation of 1 naphthol is very effective in olfactory bulb which is the pathway for entering of foreign molecules into the brain.
The main isoforms specialized in glucuronidation of phenols are:UGT 1A6 and UGT 1 A9 which preferentially catalyses the glucuronidation of small planar phenols.Literature data have shown evidence for UGT 1 A6 polymorphism exhibiting a decreased activity towards 3 O methyl dopa methylsalicilate and some beta blockers in vitro.
The aim of our study was to evaluate functional significance of olfactory glucuronidation in relation to aging using as UGT substrates different natural odorant molecules.
Material and method. Our study has been done on 30 white Wistar rats aged between: 4-37 months old : 15 controls and 15 divided into three groups of 5 rats each:group A young(4 months old), group B adult(8 month old) and group C old(37 month old).fed for 4 weeks on cholesterol reach diet 20 g/kg body weight .The activity of 1 Naphthol UGT 1A6 has been assessed in:whole brain homogenate,olfactory bulb,pituitary gland ,in choroids plexus and microvessels from young and old rats.
Results. Our data pointed out that glucuronidation process in rat brain exhibits a regional and tissular heterogeneity being increased in cholesterol feed young and old rats versus controls
Conclusion. UGTs may play a significant role in chemosensory function as well as in brain defence mechanisms against toxin accumulation.
Keywords:
Cholesterol,
Glucuronidation,
Brain,
protection
Conference:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010, Thessaloniki, Greece, 1 Oct - 5 Oct, 2010.
Presentation Type:
Poster
Topic:
Food, drugs and environmental xenobiotics
Citation:
Revnic
CR,
Atanasiu
V,
Prada
G,
Prada
S,
Pena
C,
Revnic
F and
Botea
S
(2010). The impact of cholesterol rich diet upon old Wistar rat brain metabolism.
Front. Pharmacol.
Conference Abstract:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010.
doi: 10.3389/conf.fphar.2010.60.00191
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Received:
05 Mar 2011;
Published Online:
04 Nov 2010.
*
Correspondence:
Dr. Cristian R Revnic, University of Medicine and Pharmacy”Carol Davila”, Bucharest, Romania, kityrom@yahoo.com