The influence of caffeine on aspirin passage into the central nervous system in rats
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1
Medical faculty of Novi Sad, Department of Pharmaoclogy, Toxicology and Clinical Pharmacology, Serbia
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2
Medical faculty of Novi Sad, Department of Occupational Medicine, Serbia
Introduction. In this study the effect of caffeine on aspirin passage through the blood-brain barrier(BBB) into the central nervous system(CNS) was examined.
Methods. The experiments were carried out on anaesthetized white rats(n=60); aspirin was given as a retrograde intra-arterial bolus injection(15sec) into the right axillary artery. This way of administration permitted the aspirin to be directed towards the brachiocephalic trunk, and then by blood circulation to CNS, avoiding, thus, the interference of peripheral organs to aspirin pharmacokinetics. In this study it was followed the dynamics of aspirin passage into the CNS. There were two groups of animals. To the animals of the control group it was subcutaneously given physiological solution 30min before the intra-arterial aspirin injection, whereas the animals of the test group received at the same time caffeine also subcutaneously. Caffeine and physiological solution were applied in equimolar doses. The animals were sacrificed by decapitation 30,60,90,120 and 240sec after the aspirin application. Blood samples were taken from the left jugular vein, and the brain, after rinsing, was divided into brainstem, cerebellum and cerebral hemispheres, in order to determine aspirin concentrations by standard spectrophotofluorimetric method.
Results. The aspirin concentrations were significantly lower(30 times) in the CNS than in blood what is probably a consequence of aspirin dissociation at the physiological pH value. The central kinetics of aspirin indicated that there were two CNS compartments, one consisted of the brainstem and cerebellum, and the other consisting of the cerebral hemispheres. Caffeine did not affect control group significantly the aspirin concentrations either in blood or the brain compartments, compared to the control groups of animals. Conclusion. Thus, it can he concluded that caffeine has not a significant effect upon aspirin passage through BBB into the CNS, so that the analgesic effect of aspirin cannot he enhanced by facilitating passage its passage to the CNS.
Keywords:
Caffeine,
Aspirin,
Central Nervous System
Conference:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010, Thessaloniki, Greece, 1 Oct - 5 Oct, 2010.
Presentation Type:
Poster
Topic:
Xenobiotic toxicity
Citation:
Vasovic
V,
Jakovljevic
V,
Mikov
M,
Vukmirovic
S,
Stilinovic
N and
Mikov
M
(2010). The influence of caffeine on aspirin passage into the central nervous system in rats.
Front. Pharmacol.
Conference Abstract:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010.
doi: 10.3389/conf.fphar.2010.60.00211
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Received:
28 Oct 2010;
Published Online:
04 Nov 2010.
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Correspondence:
Dr. S Vukmirovic, Medical faculty of Novi Sad, Department of Pharmaoclogy, Toxicology and Clinical Pharmacology, Novi Sad, Serbia, sasavukmirovic@neobee.net