Differential protein expression of SKOV3 ovarian cancer cells treated with standardised extract of Eurycoma longifolia (SEEL30)
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1
Forest Research Institute Malaysia (FRIM), Malaysia
Background
Ovarian cancer is the leading cause of mortality amongst women in urban countries with 225,500 new cases and 140,200 estimated deaths reported worldwide. The long-term survival of ovarian cancer patients is still poor, with less than 40% across all stages. Hence, there is an urge to search for new potential anti-ovarian cancer drug candidates. The standardised extract of E. longifolia (SEEL30) have shown active anti-cancer effect against SKOV3 ovarian cancer cell line with an IC50 of 17.26±0.11µg/mL. The acute toxicity tests showed no adverse and alarming behavioral response with LD50 > 2000mg/ kg body weight of male and female Sprague Dawley rats. Recent advances have enabled the study of cancer proteome. Proteins are the main components of the physiological metabolic pathways of cells as they provide structure, catalysing almost all the reactions that take place in a living cell. The current study therefore aimed to observe the differential expression of SKOV3 ovarian cancer cells treated with standardised extract of Eurycoma longifolia (SEEL30).
Methods
Ovarian cancer cells (SKOV3) were treated with or without (control) standardised extract of E. longifolia (SEEL30) using the IC50 value. Then, protein profiling was conducted using 2-Dimensional Electrophoresis (2-DE). The gels were stained with Coomassie Brilliant Blue-G200 (CBB-G200). Digital images of the analytical gels were acquired and analysed quantitatively for differentially expressed proteins using ImageMaster 2D Platinium 7.0 analysis software.
Results
In total, at least 900 protein spots were successfully being separated and detected in each 2-DE profiles. Only 62 proteins were however, differentially expressed in 2-DE profiles of SKOV ovarian cancer cells treated with SEEL30 as compared to the control (selection criteria: Anova p value<0.05 and protein fold change >1.5). Of the 62 differentially expressed proteins, 29 proteins were up-regulated and 33 proteins were down-regulated.
Conclusion
The differential proteins obtained will be identified and networks of the involved proteins will be elucidated.
Keywords:
2-Dimensional electrophoresis,
Proteomics,
Ovarian cancer cell line,
Standardised extract,
Eurycoma longifalia
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Cancer
Citation:
Mat Amin
N,
Yunos
NM and
Jauri
M
(2019). Differential protein expression of SKOV3 ovarian cancer cells treated with standardised extract of Eurycoma longifolia (SEEL30).
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00031
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Received:
08 Nov 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Mrs. Nor Datiakma Mat Amin, Forest Research Institute Malaysia (FRIM), Kuala Lumpur, Kuala Lumpur, Malaysia, nordatiakma@frim.gov.my
Dr. Nurhanan M Yunos, Forest Research Institute Malaysia (FRIM), Kuala Lumpur, Kuala Lumpur, Malaysia, hanan@frim.gov.my