Anti-inflammatory activity of topical polyherbal formulation containing Pereskia bleo and Camellia sinensis leaves extract
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1
Faculty of Pharmacy, Mahsa University, Malaysia
Background
Non-steroid anti-inflammatory drugs (NSAIDS) are the most prescribed drugs for the treatment of inflammatory diseases. Furthermore, prolonged use of NSAIDS is associated with severe side effects particularly on gastric mucosa, renal and cardiovascular system. Therefore, searching for new drug candidates in the treatment of chronic inflammation has great importance. Alternative to these drugs are traditional medicines and natural products, which offer a great hope in the identification of bioactive lead compounds and their development into drugs for treating inflammatory diseases. With this regard, the present study was aimed to formulate and evaluate a novel polyherbal ointment containing leaves extract of Pereskia bleo (PB) and Camellia sinensis (CS) for their anti-inflammatory activities.
Methods
Fresh air-dried leaves of PB and CS were extracted with dichloromethane using Soxhlet apparatus and subjected to phytochemical analysis. Commercially available simple ointment BP (Vaseline®) was used as a base to prepare eight polyherbal ointment formulations containing different concentrations of PB (5% and 10% w/w) and CS (1% and 2% w/w) extracts. Prepared formulations (F1 to F8) were evaluated for physical parameters along with anti-inflammatory activity by using model carrageenan-induced paw edema in rats using Diclofenac gel as a positive control.
Results
The pH of all formulations ranged between 5.50 to 6.25 which were within the acceptable pH for topical application. The paw thickness for each group was measured every hour for a total studied period of five hours. The formulation F8 has equivalently reduced the inflammation induced by carrageenan as compared to standard drug Diclofenac. The F8 reduced the inflammation by 36.79% compared to the control treated group (39.5%).
Conclusion
Based on the above observations and results, formulation F8 was found to be a promising anti-inflammatory formulation and further extensive in-vitro and in-vivo studies are warranted to evaluate its biological potency and which may be useful for further clinical applications.
Acknowledgements
Authors are thankful to MAHSA University for providing research facilities and support for the completion of this research work.
Keywords:
Pereskia bleo,
Antiinflammantory activity,
Topical formulation,
Camellia sinensins,
topical treatment,
Polyherbal ointment formulation
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Inflammatory diseases
Citation:
Qureshi
J,
Afzal
S and
Kin
LC
(2019). Anti-inflammatory activity of topical polyherbal formulation containing Pereskia bleo and Camellia sinensis leaves extract.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00120
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Received:
15 Oct 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Javed Qureshi, Faculty of Pharmacy, Mahsa University, Bandar Saujana Putra, Malaysia, javed@mahsa.edu.my