Anti-cancer potential of methyl gallate isolated from Bambangan (Mangifera pajang) in MCF-7 cell line
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1
Universiti Tun Hussein Onn Malaysia, Malaysia
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2
Putra Malaysia University, Malaysia
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3
Universiti Teknologi MARA, Malaysia
Background
Mangifera pajang or widely known as Bambangan is an indigenous fruit of Borneo Island. The kernel part of this fruit has been reported to display anti-cancer potential. This study was conducted to isolate the major bioactive compound from the kernel of Bambangan and determine the cytotoxic activity and apoptosis-inducing ability of the compound in MCF-7 cell line.
Methods
Methyl gallate (C8H8O5) has been successfully isolated from the methanol crude extract of Bambangan kernal. Identification of methyl gallate was done using spectroscopic method analysis such as infrared (IR), mass spectrometry (MS), nuclear magnetic resonance (NMR) and compared to reported data. Cytotoxic activity of methyl gallate was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The effect of methyl gallate on cell cycle and apoptosis induction was assessed using flow cytometry analysis.
Results
The results showed that methyl gallate induced strong cytotoxic activity in MCF-7 cell line with IC50 value of 54.7 µM. Methyl gallate increased cell population in sub-G1 phase indicating apoptosis and cell cycle arrest at G0/G1. In addition, staining with Annexin V-FITC and propidium iodide revealed that total apoptotic cells were increased after incubation with the compound in a time dependent manner.
Conclusion
As a conclusion, methyl gallate isolated from Bambangan induced anti-cancer potential in breast cancer cell. Study on the mechanism of action as well as the efficacy of this compound in in vivo is suggested moving forward
Acknowledgements
This study was funded under Fundamental Research Grant Scheme (Vot: 1560) as well as UTHM contract grant (H277). Special thanks to Universiti Tun Hussein Onn Malaysia (UTHM), Universiti Putra Malaysia (UPM) and Universiti Teknologi MARA (UiTM) for the use of laboratories and technical assistance.
Keywords:
Mangifera pajang,
Methyl gallate,
MCF-7,
Apoptosis,
cytotoxic
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Cancer
Citation:
Che Rahim
A,
Abu Bakar
M,
Kassim
N,
Stanslas
J and
Wan Mohamad Zain
W
(2019). Anti-cancer potential of methyl gallate isolated from Bambangan (Mangifera pajang) in MCF-7 cell line.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00149
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Received:
16 Oct 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Mohd Fadzelly Abu Bakar, Universiti Tun Hussein Onn Malaysia, Parit Raja, Malaysia, fadzelly@uthm.edu.my