Effects of Morinda citrifolia on Diabetic-Induced Renal Tissue Apoptosis
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1
Diponegoro University, Indonesia
Background
Diabetic nephropathy (DN) is a microvascular complication due to chronic hyperglycemia. Apoptosis of nephron cells are the major feature of DN. The aim of this study is to investigate the effects of Morinda citrifolia (MC) on nephron cells apoptosis due to DN.
Methods
Subjects were 30 male Sprague Dawley rats. All rats were given streptozotocin (STZ) 40 mg/kg BW to induce diabetes. The rats were randomly allocated into 5 groups based on the dose of MC extract/kg BW (MC 10 mg, 20 mg, 40 mg, 80 mg and zero extract acts as a control group). MC extracts were given at 8 weeks after STZ induction, once a day for 2 weeks. Apoptosis index (AI) was measured using TUNEL assay. Caspase-3 activities were measured using immunohistochemistry.
Results
AI of glomerular and tubular cells of MC 20, 40 and 80 mg were significantly lower than control group. AI of glomerular cells in control groups, MC10, MC20, MC40 and MC80 were 9.7±0.44, 8.9±0.89, 4.3±0.31, 6.1± 0.51, and 7.4±0.47, respectively. AI of tubular cells was 9.7± 0.47, 9.3±0.80, 4.6±0.28, 6.3±0.40, 7.6± 0.47 respectively. Caspace-3 expression of glomerular of MC20 and MC40 and tubular cells of MC 20, 40 and 80 mg also significantly lower than control. Caspace-3 expressions of glomerular cells were 3.2±0.46, 2.8±0.43, 1.4±0.27, 2.3±0.33 and 3.2±0.27, respectively. Caspace-3 expressions of tubular cells were 13.0± 0.79, 9.8±1.02, 4.8±0.72, 8.1±0.70 and 10.8±0.2 respectively. The most effective dose MC to inhibit apoptosis of nephron cells was 20 mg/kg BW.
Conclusion
MC extracts have a potency to inhibit nephron cells apoptosis due to DN.
Keywords:
Morinda citrifolia,
Diabetic Neuropathy,
apop´tosis,
TUNEL,
Caspace-3
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Oral Presentation
Topic:
Metabolic diseases
Citation:
Samsuria
IK and
Hardian
H
(2019). Effects of Morinda citrifolia on Diabetic-Induced Renal Tissue Apoptosis.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2019.63.00008
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Received:
29 Nov 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Indranila K Samsuria, Diponegoro University, Semarang, Central Java, 50275, Indonesia, nila_fkundip@yahoo.com