Tualang honey inhibits H23 human lung adenocarcinoma cell growth by modulating the expression of functional apoptotic proteins
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1
University of Malaya, Malaysia
Background
Tualang honey (TH) is a multiflora rainforest honey from Malaysia and produced by wild giant bees (Apis dorsata) that built their hive on the Tualang tree. Recently, honey has been shown to have anti-cancer properties on various cancer lines. The purpose of this study is to investigate the effect of TH on the molecular mechanism of apoptosis and proliferation in H23 human lung adenocarcinoma cell line.
Methods
3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used to evaluate the anti-proliferative effect of TH in H23 cells for 24 and 48h. Detection of apoptosis was carried out by measuring annexin V-FITC apoptosis assay. Apoptosis protein profiling was detected by RayBio® Human Apoptosis Array. The quantitative label-free LC-MS/MS strategy was performed to characterize the proteomes of H23 cell in response to TH-treatment.
Results
The MTT assay indicated that TH was able to inhibit the proliferation of H23 cells in a dose- and time-dependent manner with the IC50 value of 3.6±0.6 % and 2.7±0.2 % (v/v) for 24 and 48h, respectively. TH induced apoptosis in H23 cells by upregulating the expression of caspase-3, cytochrome c, Bad, Bax, p53 and BCLAF1 proteins (p<0.05). TH treatment significantly (p<0.05) decreased the expression of HSPB1. TH also inhibited the expression of CREB1, HDAC, PCNA and 14-3-3 proteins.
Conclusion
TH inhibited cell proliferation and enhanced apoptosis in H23 human lung adenocarcinoma cells by modulating the proteins involved in mitochondrial pathway-induced apoptosis.
Acknowledgements
This study was funded by the University of Malaya Research University grant (UMRG), RG290-14AFR and the University of Malaya Postgraduate Research Grant (PPP), PG085-2013A.
Keywords:
Tualang honey,
14-3-3 protein,
PCNA,
caspase-3,
p53
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Cancer
Citation:
Amran
N,
Abdul-Rahman
P and
Cheng
H
(2019). Tualang honey inhibits H23 human lung adenocarcinoma cell growth by modulating the expression of functional apoptotic proteins.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2019.63.00036
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Received:
30 Sep 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Puteri Shafinaz Abdul-Rahman, University of Malaya, Kuala Lumpur, Malaysia, terisar@um.edu.my