Distribution of Kisspeptin-like immunoreactivity in the human hypothalamus. Demonstration of neuronal contacts with type-1 gonadotropin-releasing hormone neurons
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1
Institute of Experimental Medicine, Hungarian Academy of Sciences, Hungary
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2
Department of Forensic Medicine, Semmelweis University, Hungary
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3
Univ Tours, INRA, France
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4
King’s College London, United Kingdom
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5
Department of Neuroscience , Pázmány Péter Catholic University, Hungary
Recent evidence demonstrates that the neuropeptide kisspeptin and its cognate receptor, GPR54, play a fundamental role in initiating the onset of puberty and regulating reproductive functions in both genders. In the present study we analyzed the distribution and relationship to GnRH1 neurons of kisspeptin containing fibers in the human hypothalamus. Hypothalamic autopsy samples from female human individuals were sectioned coronally with a freezing microtome. Kisspeptin immunoreactivity was visualized with a rabbit antiserum raised against a highly conserved 10 amino acid-amidated peptide (kp10) common to all kisspeptin isoforms, using the ABC technique and silver-intensified nickel-diaminobenzidine, as chromogen. Axon terminals showing kisspeptin-like immunoreactivity densely innervated the human infundibular stalk and the lamina terminalis. In addition, immunolabeled axon varicosities were abundant in the ventral periventricular nucleus, anteromedial and anterolateral preoptic nuclei, paraventricular nucleus (magnocellular and parvicellular subdivisions) and the infundibular nucleus. Only scattered fibers were detectable in the ventromedial and dorsomedial nuclei. A faint perikaryon labeling was noticed in the periventricular region and the infundibular nucleus. Analysis of histological preparations that were double-immunostained for kisspeptin and GnRH1 revealed kisspeptin-immunoreactive axon varicosities forming contacts with dendrites of GnRH1 neurons. Circumventricular organs of the organum vasculosum laminae terminalis and the median eminence contained overlapping networks of GnRH1- and kisspeptin-immunoreactive axons. This study presents a detailed anatomical map of kisspeptin-immunoreactive fibers in the human hypothalamus and provides light microscopic evidence for the direct innervation of human GnRH1 neurons by kisspeptin containing fibers.
Supported by OTKA (K69127, T73002) and the Sixth EU Research Framework Programme (contract LSHM-CT-2003-503041).
Conference:
12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009.
Presentation Type:
Poster Presentation
Topic:
Homeostatic regulatory mechanisms
Citation:
Hrabovszky
E,
Vida
B,
Horvath
MC,
Keller
E,
Caraty
A,
Clive
CW,
Liposits
Z and
Kalló
I
(2009). Distribution of Kisspeptin-like immunoreactivity in the human hypothalamus. Demonstration of neuronal contacts with type-1 gonadotropin-releasing hormone neurons.
Front. Syst. Neurosci.
Conference Abstract:
12th Meeting of the Hungarian Neuroscience Society.
doi: 10.3389/conf.neuro.01.2009.04.113
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Received:
03 Mar 2009;
Published Online:
03 Mar 2009.
*
Correspondence:
Erik Hrabovszky, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary, hrabovszky.erik@koki.hun-ren.hu