Reduced calcium increase after axotomy in hypoglossal motor neurons of mice with increased parvalbumin level
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1
Institute of Biophysics, Biological Research Center, Hungarian Academy Science, Hungary
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2
Department of Neurology, University of Szeged, Hungary
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3
Institute of Experimental Medicine, Hungarian Academy of Sciences, Hungary
Widely cited literature data support the idea of the protective role of certain calcium binding proteins (CaBPs) such as calbindin-D28k (CB) and parvalbumin (PV) against calcium-mediated neuronal injury. Accordingly, in our previous experiments an inverse correlation has been found between the calcium increase and PV content in hypoglossal- and oculomotor neurons after axotomy in Balb/c mice. To directly test the protective role of PV in neuronal injury in these motor nuclei, transgenic mice over-expressing rat PV were developed on B6/SJL background, using the rat CaMII promoter, and bred to homozygosity. PV+/+ mice were then introduced to axotomy/target deprivation experiments: the left hypoglossal nerve was transected lateral to the hyoid bone, and a piece of 3-5 mm was removed to prevent regeneration. To cut the left oculomotor nerve, mice were enucleated, and the orbits were cleared of the remaining extraocular muscles and nerves. Intracellular calcium was assayed with the oxalate-pyroantimonate technique electron microscopically at postoperative 1, 4, 7, 14 and 21 days. Calcium levels in injured motor neurons were expressed as fold-increase using the contralateral cells as internal reference. In the hypoglossal motor neurons of B6/SJL mice we could demonstrate a maximum of 1.7-fold calcium increase at postoperative day 7, which returned to the control level by day 21. In the PV+/+ mice there was no calcium increase either in the hypoglossal- or oculomotor neurons, suggesting that PV upregulation turned the hypoglossal neurons resistant to calcium-mediated injury.
Supported by OTKA (T-048718), GVOP (2004-04-0052/3.0) and RET-DNT (08/04).
Conference:
12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009.
Presentation Type:
Poster Presentation
Topic:
Pathophysiology and neurology - degenerative disorders
Citation:
Paizs
M,
Engelhardt
J,
Katarova
Z,
Gyenes
A and
Siklos
L
(2009). Reduced calcium increase after axotomy in hypoglossal motor neurons of mice with increased parvalbumin level.
Front. Syst. Neurosci.
Conference Abstract:
12th Meeting of the Hungarian Neuroscience Society.
doi: 10.3389/conf.neuro.01.2009.04.170
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Received:
05 Mar 2009;
Published Online:
05 Mar 2009.
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Correspondence:
Laszlo Siklos, Institute of Biophysics, Biological Research Center, Hungarian Academy Science, Szeged, Hungary, siklos@brc.hu