A combined approach to unravel single cell function using complex visual stimuli, intracellular staining and intrinsic signal optical imaging in the cat primary visual cortex
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1
Department of Anatomy, Histology and Embriology, University of Debrecen, Hungary
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2
Unite de Neuroscience Intégratives et Computationnelles, CNRS, France
In the primary visual cortex (V1), cortico-cortical feed-back connections, which derive from higher order cortical areas, are assumed modulate the response properties of V1 neurons. Although feed-back connections represent a substantial proportion of all input to V1 their functional significance and termination pattern of synapses at the single cell level remains elusive. Here we used brain imaging, electrophysiological and morphological approaches to reveal the contribution of feed-back connections of area 18 (A18) to neurons in area 17 (A17). The border zone between A17 and A18 was determined using activity maps obtained by intrinsic signal optical imaging based on the different spatial and temporal frequency preferences between A17 and A18 neurons. In A17, close to the border zone with A18, intracellular recordings and tracer injections (biocytin) were made from layer 2/3 pyramidal cells using a battery of quantitative test for spatial frequency, orientation, motion, direction, velocity (thus temporal frequency), and many other spatiotemporal features. In A18, multi unit activity was recorded from several locations (1-4 mm from the A17 recording/injection sites) and test stimuli employed for determining preferred orientation, direction and retinotopic position. Finally, extracellular injection of BDA was carried out at each electrode pentration to visualize feed-back connections to A17 neurons. Labelled pyramidal cells in A17 receiving labelled axons from A18 were reconstructed in three-dimension. Our combined method enables us (i) to determine the synaptic placement and distribution of feed-back input on V1 neurons and (ii) relate their role in processing complex visual stimuli.
Supported by the European Consortium, FACETS (FP6-2004-IST-FETPI) and the Hungarian Academy of Sciences (TKI-242) to Z.K.
Conference:
12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009.
Presentation Type:
Poster Presentation
Topic:
Research on the cerebral cortex and related structures
Citation:
Sari
K,
Fuyuki
K,
Fournier
J,
Cyril
M,
Fregnac
Y and
Kisvarday
Z
(2009). A combined approach to unravel single cell function using complex visual stimuli, intracellular staining and intrinsic signal optical imaging in the cat primary visual cortex.
Front. Syst. Neurosci.
Conference Abstract:
12th Meeting of the Hungarian Neuroscience Society.
doi: 10.3389/conf.neuro.01.2009.04.202
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Received:
06 Mar 2009;
Published Online:
06 Mar 2009.
*
Correspondence:
Katalin Sari, Department of Anatomy, Histology and Embriology, University of Debrecen, Debrecen, Hungary, sari.katalin@gmail.com