The role of myosin phosphatase in the synaptic transmission in the rat ventral cochlear nucleus
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1
Departments of Physiology , Medical and Health Science Centre, University of Debrecen, Hungary
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2
Departments of Medical Chemistry, Medical and Health Science Centre, University of Debrecen , Hungary
It has been suggested that myosin phosphatase (MP) may have a regulatory role in neurotransmission. In this work involvement of MP was investigated in the synaptic action occurring in a mammalian giant synapse (endbulbs of Held) situated in the rat ventral cochlear nucleus (VCN). Whole-cell measurements were performed on bushy neurones using a thin-slice preparation. To evoke the activation of the glutamatergic synapse situated between the the acoustic nerve fibers and the cell bodies of the bushy cells, the root of the auditory nerve was stimulated (50 Hz), and the resulting excitatory postsynaptic currents (EPSCs) were recorded. To assess the significance of the MP, the effects of tautomycetin (a protein phosphatase-1-specific inhibitor) and Y27632 (a rho-kinase-specific inhibitor) were determined on various parameters of the EPSCs. When the slices were pre-incubated in 5 µM tautomycetin, the amplitude of the first EPSC was decreased, whereas the relative amplitude of the subsequent EPSCs was increased. The decay tau of the EPSCs was also significantly increased. In contrast, exactly the opposite actions have been noted in the case of Y27632 application (10 µM): the amplitude of the first EPSC increased, the relative amplitudes of the subsequent EPSCs reduced, and the decay tau was increased. Our results suggest that MP has important roles in the regulation of the synaptic transmission occurring in the giant synpse ofthe VCN, and the regulatory effect is exerted via both pre and postsynaptic mechanisms.
Supported by the OTKA (K-68416 and K-72812)
Conference:
12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009.
Presentation Type:
Poster Presentation
Topic:
Developmental neurobiology and subcortical functions
Citation:
Kőszeghy
Á,
Pál
B,
Szűcs
G,
Lontay
B,
Erdodi
F and
Rusznak
Z
(2009). The role of myosin phosphatase in the synaptic transmission in the rat ventral cochlear nucleus.
Front. Syst. Neurosci.
Conference Abstract:
12th Meeting of the Hungarian Neuroscience Society.
doi: 10.3389/conf.neuro.01.2009.04.236
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Received:
13 Mar 2009;
Published Online:
13 Mar 2009.
*
Correspondence:
Áron Kőszeghy, Departments of Physiology , Medical and Health Science Centre, University of Debrecen, Debrecen, Hungary, aron.koeszeghy@meduniwien.ac.at