Blood vessels are required for development of the enteric nervous system
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1
Department of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, United States
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2
Department of Human Morphology and Developmental Biology , Semmelweis University , Hungary
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3
National Institutes of Child Health and Human Development, United States
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4
National Center for Biotechnology Information, National Institutes of Health, United States
Enteric neural crest-derived cells (ENCCs) migrate along the intestine to form a highly organized network of ganglia that comprises the enteric nervous system (ENS). The signals driving the directed migration of these cells are largely unknown. We examined the intestinal neurovasculature in avian embryos and find that endothelial cells are present in the gut prior to ENCC arrival and are patterned in concentric rings predictive of the patterning of arriving crest-derived cells. ENCCs migrate adjacent to endothelial cells as they colonize the gut in both avians and zebrafish. Inhibition of endothelial cell development in avian intestine leads to aganglionosis, suggesting that ENCC migration requires the presence of endothelial cells. Tissue explant experiments were used to demonstrate that vagal-derived neural crest cells will migrate onto a surface of cultured endothelial cells. Similarly, when embryonic midgut is explanted onto an endothelial cell surface, ENCCs migrate out of the gut and onto the surrounding endothelial substrate. This migratory effect of endothelial cells is inhibited by the presence of CSAT antibody, which interferes with binding to b1 integrins, shown to be expressed on the surface of ENCCs. These results suggest that interactions between endothelial cell-derived ECM molecules and 1 integrin-expressing ENCCs are required for migration of crest-derived cells during development of the ENS.
Conference:
12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009.
Presentation Type:
Poster Presentation
Topic:
Developmental neurobiology and subcortical functions
Citation:
Nagy
N,
Mwizerwa
O,
Yaniv
K,
Carmel
L,
Vanmarcke
RP,
Weinstein
BM and
Goldstein
AM
(2009). Blood vessels are required for development of the enteric nervous system.
Front. Syst. Neurosci.
Conference Abstract:
12th Meeting of the Hungarian Neuroscience Society.
doi: 10.3389/conf.neuro.01.2009.04.247
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Received:
13 Mar 2009;
Published Online:
13 Mar 2009.
*
Correspondence:
Nándor Nagy, Department of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, United States, nagyn@ana2.sote.hu