Effects of pesticide thiamethoxan, and its metabolite clothianidin,
on in vivo dopamine release in rat striatum.
-
1
Universidad de Vigo, Spain
Background:Thiamethoxam (TMX) and its principal metabolite clothianidin (CLO), with high deposition in brain, are synthetic organic insecticides included in the class of neonicotinoids, a worldwide used pesticide and the most important new class of insecticides developed in the past three decades. Although it is clear the data about the action of neonicotinoid pesticides on insect nAChRs, there are few works worried in showing possible in vivo effects of this group of pesticide upon mammalian biologic system.
Objective:The objective of this study was to characterize the effects of TMX and CLO on in vivo release of dopamine (DA) of rat striatum using brain microdialysis in freely moving animals.
Methodology:TMX and CLO, at different concentrations, have been administrated during a period of 1 h, directly in striatum of female Sprague-Dawley adult rats (250-300g, 5/group) through a microdialysis probe. DA levels, sampled by microdialysis, have been measured by HPLC-EC. The statistical analysis was made by ANOVA/Student-Newman-Keuls test. Considered differences:
P<0,05 and P<0,01.
Results:Intrastriatal administration of TMX at the concentrations of 1, 5 and 10 mM has produced significant increases on extracellular DA levels: 159,3±20,17%, 165,54±21,05% and 840,96±131,64%, compared to basal levels, respectively. Intrastriatal administration of CLO has produced increases in extracellular DA levels: 1 mM: 225,45 ± 64,19%; 2 mM: 437,65 ± 83,64%; 3,5 mM: 2778,52 ± 597,87%; 5 mM: 4604,19 ± 515,77%; and 10 mM: 9700 ± 2035,75%, every compared to basal levels.
Conclusion:These results show that intrastriatal infusion of TMX induce increases in extracellular DA levels in rat striatum, being these increases concentration-dependent. On the other hand, intrastriatal administration of CLO also induced increases in extracellular DA levels, but these increases seem not to be concentration-dependent.
Finantial support. Xunta de Galicia, Universidad de Vigo.
Conference:
11th Meeting of the Portuguese Society for Neuroscience, Braga, Portugal, 4 Jun - 6 Jun, 2009.
Presentation Type:
Poster Presentation
Topic:
Neuronal Communication
Citation:
Oliveira
IM,
Viviane-Ferreira-Nunes
B,
Cervantes
RC,
Alfonso
M,
Duran
R and
Faro
LR
(2009). Effects of pesticide thiamethoxan, and its metabolite clothianidin,
on in vivo dopamine release in rat striatum..
Front. Neurosci.
Conference Abstract:
11th Meeting of the Portuguese Society for Neuroscience.
doi: 10.3389/conf.neuro.01.2009.11.153
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Received:
12 Aug 2009;
Published Online:
12 Aug 2009.
*
Correspondence:
Brenda Viviane-Ferreira-Nunes, Universidad de Vigo, Vigo, Spain, brendaneuro@hotmail.com