Curcumin and Tetrahydrocurcumin protects mouse brain from neurotoxicity and oxidative stress caused by 1 – methyl – 4 – phenyl – 1, 2, 3, 6 – tetrahydropyridine induced Parkinsonism
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1
Annamalai University, Division of Neuroscience, Department of Zoology, India
We investigated the effects of the polyphenolic compound curcumin and its metabolite tetrahydrocurcumin, in the model of Parkinson’s disease induced in mice by 1 – methyl – 4 – phenyl – 1, 2, 3, 6 – tetrahydropyridine (MPTP). Curcumin is the principal yellow pigment extracted from turmeric, a food additive using in India and China. Tetrahydrocurcumin (THC) is one of the major metabolite of curcumin, which has potent bioactivity. MPTP is a potent neurotoxin that produced nigral dopaminergic neuronal damage in humans, primates and rodents. In the brain, MPTP is converted to its biologically active metabolite1-methyl 1-4 phenyl pyridinium (MPP+) by the action of monoamine oxidase- B (MAO-B) which is toxic to neurons. Animals with low levels of MAO-B in the brain are resistant to this neurotoxin. Adult male Swiss albino mice (25-30 g) were used in this study. In the present study, four injections of MPTP were administered at the dose of 10mg /kg (i.p) with 1 hr intervals totaling of dose 40mg/kg/day.Curcumin and THC were treated for animals with 80mg/kg/day and 60 mg/kg/day for 7 days respectively. All the protocols were followed on the 3rd and 7th day of treatment. The locomotory effect and neuromuscular ability were assessed by Narrow beam walk test and Hang test. The MPTP induced significant increase in time on the 3rd and 7th day of treatment. The treatment of curcumin and Tetrahydrocurcumin significantly reverse the time. We measured the (GSH) reduced glutathione, (CAT) catalase and (SOD) superoxide dismutase activity in the (ST) Striatum and (MB) midbrain 3rd and 7th day following MPTP and curcumin and THC administration. MPTP treatment caused a significant depletion in GSH and increase the specific activity of SOD, CAT, and lipid per oxidation in ST and MB. Curcumin exhibited a synergistic effect on SOD and CAT activities in the ST and MB regions. In this model depletion of DA (Dopamine) and DOPAC occurs with increase monoamine oxidase (MAO-B) activity. The treatment of Curcumin and THC significantly reversed the MPTP induced depletion of DA and DOPAC. The MAO-B activity was also significantly inhibited by these compounds. The results showed that the curcumin and THC reversed the MPTP induced depletion of DA and DOPAC, which may in part be due to the inhibition of MAO-B activity. The phenolic OH is essential for both antioxidant activity and free radical kinetics. In conclusion, both curcumin, and THC exert neuroprotection against oxidative stress by MPTP induced neurotoxicity. Strikingly the THC has elicited more antioxidant activity when compared to curcumin.
Conference:
3rd Mediterranean Conference of Neuroscience , Alexandria, Egypt, 13 Dec - 16 Dec, 2009.
Presentation Type:
Oral Presentation
Topic:
Neurobiology of pain and depression
Citation:
Muthukumar
S and
Arulkumar
S
(2009). Curcumin and Tetrahydrocurcumin protects mouse brain from neurotoxicity and oxidative stress caused by 1 – methyl – 4 – phenyl – 1, 2, 3, 6 – tetrahydropyridine induced Parkinsonism.
Front. Neurosci.
Conference Abstract:
3rd Mediterranean Conference of Neuroscience .
doi: 10.3389/conf.neuro.01.2009.16.121
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Received:
23 Nov 2009;
Published Online:
23 Nov 2009.
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Correspondence:
Sabesan Muthukumar, Annamalai University, Division of Neuroscience, Department of Zoology, Tamilnadu, India, sabesan1956@gmail.com