Toxic effects of lead exposure on the brain of rats: involvement of oxidative stress, inflammation, acetylcholinesterase and the beneficial role of flaxseed extract
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1
Alexandria University, Biochemistry Department, Egypt
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2
Mubarak City for Scientific Research, Department of Protein Technology, Egypt
The current study was carried out to investigate the effects of Pb exposure at low levels on brain tissue antioxidant enzymes activities and acetylcholinesterase (AChE), inflammatory markers (nitrites (NO) and TNF – α), and lipid profile. Furthermore, we also investigated the possible effects of flaxseed extract to revise the lead toxicity. For that sake, Female Sprague-Dawelly rats were exposed to lead (200 mg Pb/l in drinking water) for three weeks followed by 21 days of orally administrated flaxseed extract (300 mg/kg). AChE activity increase by 64 % and a significant decrease in glutathione level, total antioxidants capacity, glutathione – S – transferease, and superoxide dismutase and catalase activities after lead exposure. Moreover, NO and α – TNF were increased by 166.5 % and 400 %, respectively. Finally, lead exposure increased the brain lipid profile of cholesterol and triglycerides. Chronic treatment with flaxseed significantly attenuated cholinergic dysfunction, oxidative stress and inflammation in brain tissue after three-week treatment period. All together, the result emphasized the involvement of different factors such as of oxidative stress, inflammation, and high expression of AChE activity in lead – neurotoxicity and showed flaxseed as a new adjuvant therapy to prevent lead – neurotoxicity.
Key words: Lead toxicity, lipid peroxidation, Acetylcholinesterase
Conference:
3rd Mediterranean Conference of Neuroscience , Alexandria, Egypt, 13 Dec - 16 Dec, 2009.
Presentation Type:
Poster Presentation
Topic:
Stress
Citation:
Ghareeb
D and
Khalil
A
(2009). Toxic effects of lead exposure on the brain of rats: involvement of oxidative stress, inflammation, acetylcholinesterase and the beneficial role of flaxseed extract.
Front. Neurosci.
Conference Abstract:
3rd Mediterranean Conference of Neuroscience .
doi: 10.3389/conf.neuro.01.2009.16.133
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Received:
24 Nov 2009;
Published Online:
24 Nov 2009.
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Correspondence:
Doaa Ghareeb, Alexandria University, Biochemistry Department, Alexandria, Egypt, hadiergad@yahoo.com