Differences in effects of oxytocin and carbetocin on stress-induced behavior of Wistar rats
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1
Charles University in Prague, Faculty of Medicine, Czechia
Neuropeptide oxytocin (OXY) has many central regulatory functions including regulation of behavior and cognition; it is assumed that its role is also involved in the regulation of stress responses. Due to these effects OXY and its analogs are intensively investigated as potential remedies for treatment of various psychiatric disturbances where OXY may participate on the etiology of these disorders. Oxytocin analog carbetocin (CBT; 1-butanoic acid-2-[O-methyl-L-tyrosine]1-carba-oxytocin) was introduced as a long-lasting drug for treatment of gynecological disturbances, however, its central effects are not sufficiently known. The aim of this study was the comparison of behavioral effects of OXY and CBT in rats exposed to acute stress, which deteriorates some behavioral parameters in open-field test (activity, exploration, and anxiety). In Wistar rats (males, 280-300 g) we applied immobilization stress lasting 60 min. OXY or CBT (1 mg/kg b.w.) or saline were given i.p. in a volume of 2 ml/kg b.w. immediately after stress termination. Behavioral testing in the open-field device was performed one hour after stress and/or drug treatment. We used circular arena (150 cm diameter), and behavioral parameters were recorded by AnyMaze software (Stoelting Co., USA). The results were evaluated by one-way ANOVA. When compared to controls, stress reduced horizontal as well as vertical (rearing) exploratory activity and increased anxiety measured by the entry to the inner zone. OXY and CBT produced diverse effects. OXY alone increased very potently grooming and did not produce any significant changes after stress. CBT, which does not produce grooming, antagonized some deteriorating effects of stress-induced behavior. These effects were long-lasting and survived for several days. Repeated application of CBT increased its effects. Revealed effects of CBT can be considered as antistress actions with potential therapeutic application.
Supported by grant of MSM 0021620806.
Conference:
41st European Brain and Behaviour Society Meeting, Rhodes Island, Greece, 13 Sep - 18 Sep, 2009.
Presentation Type:
Poster Presentation
Topic:
Poster presentations
Citation:
Klenerova
V and
Hynie
S
(2009). Differences in effects of oxytocin and carbetocin on stress-induced behavior of Wistar rats.
Conference Abstract:
41st European Brain and Behaviour Society Meeting.
doi: 10.3389/conf.neuro.08.2009.09.194
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Received:
10 Jun 2009;
Published Online:
10 Jun 2009.
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Correspondence:
Vera Klenerova, Charles University in Prague, Faculty of Medicine, Prague, Czechia, vera.klenerova@LF1.cuni.cz