Voltage-sensitive dye imaging of visually evoked responses in the goldfish telencephalic pallium
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1
Universidad de Sevilla, Laboratorio de Psicobiologia, Spain
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2
Universidad de Huelva, Spain
Information about the sensory areas in the telencephalon of teleosts is necessary not only for identifying sensory organization in fishes, but also for understanding the evolution of pallium anatomy and functional organization through vertebrate phylogeny. Nowadays numerous anatomical and a few electrophysiological studies have clarified that different sensory modalities reach different targets within the area dorsalis. For instance numerous hodological studies have revealed that in different representative species of teleost fish, the visual information from the optic tectum projects mainly to the dorsal part of the lateral pallium (Dld), via the preglomerular nuclei. However, functional studies aimed to describe the location and physiology of the teleost fish visual pallium are still scarce. In the present work we studied in vivo the pattern of visually evoked activity in the telencephalic pallium of goldfish (Carassius auratus) using the optical recording technique. For this purpose, the telencephalon was stained with a voltage sensitive dye (Di-2-ANEPQ) and the neural response evoked by the visual stimuli recorded with a CCD-based digital high-speed camera system and epi-fluorescent optics. The analysis of the spatio-temporal pattern of activity showed that visual stimulation evoked a depolarization response located in the dorsal part of the lateral pallium and confirms the visually driven-activity of neurons in this area. In addition to the size, shape and location of the visual pallium, we also describe some of its physiological characteristics as the latency, and time to reach the peak of activity.
Conference:
41st European Brain and Behaviour Society Meeting, Rhodes Island, Greece, 13 Sep - 18 Sep, 2009.
Presentation Type:
Poster Presentation
Topic:
Poster presentations
Citation:
Ocana-Campos
F,
Duran
E,
Rodriguez
F and
Salas
C
(2009). Voltage-sensitive dye imaging of visually evoked responses in the goldfish telencephalic pallium.
Conference Abstract:
41st European Brain and Behaviour Society Meeting.
doi: 10.3389/conf.neuro.08.2009.09.249
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Received:
12 Jun 2009;
Published Online:
12 Jun 2009.
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Correspondence:
Francisco-Manuel Ocana-Campos, Universidad de Sevilla, Laboratorio de Psicobiologia, Sevilla, Spain, fmocana@us.es