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Fabrication of thin transparent collagen film using collagen extract from pig skin

Kyung Mi Shim1, Se Eun Kim1, Sun-Jeong Park1 and Seong Soo Kang1
  • 1 Chonnam National University, Department of Veterinary Surgery, Korea

Introduction: Collagen is one of the most studied and used biomolecules in tissue engineering because of its high biocompatibility, biodegradability, and low antigenicity. However, its wide application in tissue engineering is limited since collagen is obtained through a quite complicated extraction process and exhibits poor mechanical and enzymatic resistance. In this study, we extracted type I collagen from pig skin and produced thin transparent collagen film after crosslinking.

Materials and Methods: Type I atelocollagen was extracted from pig skin using acetic acid in the presence of pepsin, and then purified by dialysis against distilled water and lyophilized. The collagen purity was assessed by SDS-PAGE and collagen assay kit. Extracted collagen was crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) with N-hydroxysuccinimide (NHS). Thin transparent collagen film was produced by air-drying of crosslinked or non-crosslinked collagen solution.

Results and Discussion: The extracted collagen contained two α-chains and one β-chain, and the purity was similar to that of the FDA approved collagen (Atelocollagen, Tissen, Republic of Korea). Both thin collagen film fabricated from crosslinked or non-crosslinked collagen were durable and transparent. Moreover, their characteristics were maintained well even in PBS for 7 days.

Conclusions: We could set up a simple but effective collagen extraction procedure from pig skin. Additionally, we fabricated thin transparent collagen film and evaluated its stability. While more studies are required, this study will allow the next steps in the development of a corneal bandage and a tissue-engineered cornea.

This research was supported by a grant of the Commercializations Promotion Agency for R&D Outcomes (COMPA) funded by the Ministry of Science, ICT and Future Planning(MISP), the Industrial Technology Innovation Program (No. 10048358) funded by the Ministry Of Trade, Industry & Energy (MI, Korea), and a grant from the Next-Generation BioGreen 21 Program (No. : PJ01135201), Rural Development Administration, Republic of Korea.

References:
[1] J. Yoshida et. al. Invest Ophthalmol Vis Sci. 55: 4975–4981, 2014

Keywords: Regenerative Medicine, biomaterial, Biodegradable material

Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.

Presentation Type: Poster

Topic: Biomaterials for ophthalmic applications

Citation: Shim K, Kim S, Park S and Kang S (2016). Fabrication of thin transparent collagen film using collagen extract from pig skin. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00369

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Received: 27 Mar 2016; Published Online: 30 Mar 2016.