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In vitro cytotoxicity of silver nanoparticles as a hydroxyapatite modifier on human fibroblasts

Agnieszka Sobczak-Kupiec1, Klaudia Pluta1, Dagmara Malina1, Katarzyna Bialik-Wąs2 and Bożena Tyliszczak2
  • 1 Institute of Inorganic Chemistry and Technology, Cracow University of Technology, Poland
  • 2 Department of Chemistry and Technology of Polymers, Cracow University of Technology, Poland

Biocompatible, bioactive and osteoconductive hydroxyapatite of general formula [HAp, Ca10(PO4)6(OH)2] is the most promising material used widely in medicine. Notably, HAp is extensively used as a material for regeneration of tissue defects caused by resection of the tumor or fractures as a consequence of osteoporosis as well as in dentistry. Hydroxyapatites modified with silver nanoparticles of enhanced antibacterial properties are of great interest in the development of new products.

The main goal of this study was to evaluate the potential toxic cellular effects of different concentration of silver nanoparticles suspension on human fibroblasts. The following materials were used in experiments: fibroblast cell line, BJ (ATCC, CRL-2522TM, from the foreskin of newborn with PN-EN ISO 10993-5:2009 standards which was grown in DMEM supplemented with fetal calf serum (FBS, 10% (v/v). Cytotoxicity was evaluated with MTT assay. Silver nanoparticles were synthesized by chemical reduction using aqueous solution of polyvinylpyrrolidone as a stabilizing agent.

Sample denoted as 1 is comprised of silver nanoparticles where sodium borohydride was used as reducing agent, samples 2 denotes 1:1 ratio between silver ions and reducing agent and accordingly sample 3 denotes 1:10 ratio between silver ions and reducing agent.

The Figure 1 shows the results of suspensions of silver nanoparticles exposed to cell viability dermis after 24 hours incubation in 37˚C. Figure 1 exemplify also correlation between relative values of samples determined in respect to control samples. The viability of cells for each sample is expressed as relative value estimated with respect to sample that contain the suspension of nanoparticles.

Figure 1. Viability of fibroblasts after 24h exposure to varying concentrations of AgNPs determined by MTT assay

The study showed the correlation between concentration of silver nanoparticles  and  viability of dermis cells. With increasing concentrations of AgNPs suspensions, cell viability decreases. The analysis  showed no significant differences in viability of cells when using different reducing agents for synthesis of silver nanoparticles.

This work was financed by the National Centre for Research and Development under the Lider project contract no. 037/481/L-5/13/NCBR/2014

References:
[1] Rumpel E., Wolf E., Kauschke E., Bienengräber V., Folia Morphol. Vol. 65, No. 1, pp. 43–48.

Keywords: in vitro, cell, Cell response

Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.

Presentation Type: Poster

Topic: Regeneration inducing biomaterials

Citation: Sobczak-Kupiec A, Pluta K, Malina D, Bialik-Wąs K and Tyliszczak B (2016). In vitro cytotoxicity of silver nanoparticles as a hydroxyapatite modifier on human fibroblasts. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00621

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Received: 27 Mar 2016; Published Online: 30 Mar 2016.