Promotion of osteogenesis and biomineralization in mouse mesenchymal stromal cells by collagen nanofiber modified 3D printed beta- TCP scaffold
-
1
Schoolf of Materials and School of Materials Sci & Engi, South China University of Technology, Department of Biomedical Engineering, China
-
2
National Engineering Research Center for Tissue Restoration and Reconstruction, China
Introduction: Well-defined 3D architecture of the bone graft, especially the proper interconnected pore structure, is required to allow for new bone in-growth, vascularization and degradation approaches, which may result in suboptimcal bone regeneration. In the present study, we fabricated collagen nanofiber modified 3D beta-TCP printed scaffolds aiming for an enhanced osteogenesis and biomineralization.
Materials and Methods: Scaffolds were fabricated via 3D printing of β-TCP powder using an optimized aqueous binder. Collagen Ⅰ gel were coated on the β-TCP scaffolds post sintering process. The scaffolds were characterized by SEM, Micro-CT and ICP analysis. mMSCs were seeded on the scaffolds for in vitro study. CCK-8, qPCR, mechanical testing, immunostaining, SEM and TEM were used in this study to evaluate the osteoinductivity of the scaffolds.
Results and Discussion: Our results demonstrated that both printed β-TCP scaffold and collagen gel coated scaffold exhibited excellent mechanical properties and supported the growth of mouse mesenchymal stromal cells. Meanwhile, the osteogenic phenotypic properties of mMSCs on the printed scaffolds and collagen gel coated scaffolds were investigated via ALP activity assay, molecular analysis, SEM and TEM. Results showed that the thin layer of biomimetic collagen nanofiber on printed β-TCP scaffold significantly stimulated the osteoblastic differentiation by up-regulating the expression of ALP, Runx-2, BMP2, collagen Ⅰ, OPN and BSP. In addition, a more active biogenesis of matrix vesicle was observed in mMSCs on collagen gel coated scaffolds, evidenced by SEM and TEM images together with significant higher gene expression of matrix vesicle components, indicating an enhanced initial of biomineralization.
Conclusion: In summary, we fabricated β-TCP bone scaffold with well-defined inter-connective pores via directly 3D printing technology. After hardening the printed scaffold with sintering process, Recombinant type Ⅰ collagen gel were coated on the surface of the β-TCP scaffold post sintering to form a secondary ultrafine fibrous network similar to the natural collagen nanofiber in bone. While both scaffolds exhibited excellent mechanical properties and biocompatibility, the thin layer of biomimetic collagen nanofiber on printed β-TCP scaffold significantly enhanced the osteoblastic differentiation in mMSCs and the matrix mineralization. Our study not only provided a novel 3D printed β-TCP scaffold for bone repair, but also highlight the significance of corporation of collagen nanofibrous structure in the graft to better mimicking the native bone.
National Basic Research Program of China (Grant No. 2012CB619100); the National Natural Science Foundation of China (Grant No. 51402108)
Keywords:
Cell Differentiation,
3D scaffold,
Bone graft,
matrix-cell interaction
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Biomaterials in musculoskeletal orthopeadics and tissues
Citation:
Fu
X,
Zou
F,
Zhao
N and
Wang
Y
(2016). Promotion of osteogenesis and biomineralization in mouse mesenchymal stromal cells by collagen nanofiber modified 3D printed beta- TCP scaffold.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.01009
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
27 Mar 2016;
Published Online:
30 Mar 2016.
*
Correspondence:
Dr. Xiaoling Fu, Schoolf of Materials and School of Materials Sci & Engi, South China University of Technology, Department of Biomedical Engineering, Guangzhou, China, Email1
Dr. Fen Zou, Schoolf of Materials and School of Materials Sci & Engi, South China University of Technology, Department of Biomedical Engineering, Guangzhou, China, Email2
Dr. Naru Zhao, Schoolf of Materials and School of Materials Sci & Engi, South China University of Technology, Department of Biomedical Engineering, Guangzhou, China, Email3