Accumulation of tolerogenic cells at the site of granulomatous foreign-body reaction
Dragana
Vucevic1, 2*,
Sasa
Vasilijic1, 2,
Jelena
Djokic1,
Biljana
Bozic3,
Ivana
Majstorovic1,
Sergej
Tomic2,
Milijana
Vasiljevska1,
Biljana
Draskovic-Pavlovic1, 2 and
Miodrag
Colic2
-
1
Institute for Medical Research Military Medical Academy, Serbia
-
2
Medical Faculty of the Military Medical Academy, University of Defense, Serbia
-
3
Faculty of Biology, Institute for Physiology and Biochemistry, Serbia
The granulomatous foreign-body reaction (GFBR) is a special type of chronic inflammation characterised by infiltration of inflammatory cells (ICs). Using a model of subcutaneous implantation of polyvinyl sponges in rats, we studied the phenotypic and functional characteristics of these cells, with particular reference to the properties of antigen presenting cells and lymphocytes. ICs were isolated from sponge exudates at days 1-21 after implantation. Dendritic cells (DCs) and lymphocytes were purified using a combination of separation gradients, adherence to plastics, and immunomagnetic sorting. We showed that dominant population of ICs at day 1 and 3 were granulocytes, followed by their decrease thereafter. The number of mononuclear cells (MNCs), macrophages and DCs, progressively increased after day 3, reaching maximal values at day 7. Maximal number of lymphocytes was detected at day 10. In addition, total ICs isolated from day 7 till day 10 exerted significant suppressive activity in co-culture with autologous ConA-stimulated thymocytes and alogeneic lymph node T cells. This finding correlated with the increased level of IL-10 in culture supernatants and the increased proportion MHC class II+ IDO+ DCs and CD3+ CD25+ Foxp3+ lymphocytes at day 7 and day 10, respectively. We did not find any significant difference in the number of these cell populations between regional lymph nodes MNCs and peripheral blood MNCs of experimental animals compared to controls. In conclusion, accumulation of cells with tolerogenic characteristics in GFBR might be relevant for the suppression of inflammation and the prevention of unwanted immune response.
Keywords:
granulomatous foreign-body reaction,
Dendritic Cells,
regulatory T cells,
Lymph Node,
chronic inflammation
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Adaptive Immunity
Citation:
Vucevic
D,
Vasilijic
S,
Djokic
J,
Bozic
B,
Majstorovic
I,
Tomic
S,
Vasiljevska
M,
Draskovic-Pavlovic
B and
Colic
M
(2013). Accumulation of tolerogenic cells at the site of granulomatous foreign-body reaction.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00029
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Received:
08 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Prof. Dragana Vucevic, Institute for Medical Research Military Medical Academy, Belgrade, Serbia, draganavucevic@yahoo.com