The neurobehavioral development in PACAP knockout mice
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1
University of Pécs, Department of Anatomy, Hungary
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2
University of Pécs, Department of Pharmacology and Pharmacotherapeutics, Hungary
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3
Osaka University, Graduate School of Pharmaceutical Sciences, Japan
Pituitary adenylate cyclase activating polypeptide (PACAP) is a neurotrophic and neuroprotective peptide playing important roles during the development of the nervous system. PACAP deficient mice have been shown to display several abnormalities, including alteration of cerebellar structure, behavioral abnormalities and cognitive deficits. The aim of the present study was to investigate whether the neurobehavioral development is affected in PACAP knockout mice. Somatic and neurobehavioral development was tested daily during the first 3 weeks (appearance of negative geotaxis, sensory reflexes, righting reflexes, development of fore- and hindlimb grasp and placing, gait and auditory startle reflexes). Time to perform negative geotaxis, surface righting and gait reflexes was also measured. Motor coordination tests were performed on postnatal weeks 3-5. An open-field test was performed on weeks 4-8. Our results show that the development of the major neurological reflexes was not significantly altered in the knockout mice. The motor coordination tests and open field test showed a hyperactivity in KO mice, but the other signs were not altered. We have previously shown that the battery of tests used in the present study sensitively shows alterations induced by postnatal injuries, such as asphyxia, hypoxia and maternal deprivation. Based on the present findings, it seems that the endogenous deficiency in PACAP does not cause major developmental delay. This is also in accordance with other observations where PACAP deficient mice have been shown to have normal organ development but they are much more sensitive to stress- or harmful stimuli.
Support: OTKAF67830, K72592,73044, CNK78480, ETT, PTE AOK Research Grant 2009, Bolyai Scholarship, Richter Foundation, Japan Society for the Promotion of Science.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Development
Citation:
Horváth
G,
Farkas
J,
Reglodi
D,
Helyes
Z,
Hashimoto
H,
Baba
A,
Horváth
Z,
Matkovits
A,
Tamás
A and
Kiss
P
(2010). The neurobehavioral development in PACAP knockout mice.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00012
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Received:
16 Apr 2010;
Published Online:
16 Apr 2010.
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Correspondence:
Gábor Horváth, University of Pécs, Department of Anatomy, Pécs, Hungary, figgol@freemail.hu