Retinoid metabolism and retinoic acid signaling in astrocytes and differentiating neural stem cells
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1
Hungarian Academy of Science, Institute of Experimental Medicine, Hungary
Retinoic acid (RA) is widely used as a potent inducer of neuronal differentiation by various multipotent cell populations (embryonic carcinoma cells, embryonic and neural stem cells), in vitro. The in vitro studies suggest that RA may function as a regulator of neural stem cell fate in vivo. This implication is supported by earlier, as well as recent findings on retinoid signaling at sites of both embryonic and postnatal neurogenesis. In the present study, we explore the components of retinoid metabolism/signaling in differentiating neural stem cells as well as in astroglial cells isolated from hGFAP-GFP mice. Our recent data show, that the key enzymes of RA synthesis, the retinaldehyde dehydrogenases (RALDHs) are upregulated during neuronal differentiation. In line with this, RA production is elevated in the course of neuronal/glial differentiation. Based on earlier findings (Kornyei et al. 2007, FASEB J. 21(10):2496-509) we monitor vitamin A storing and retinoic acid producing capacity of both cultured and FACS-sorted astroglial cells.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Development
Citation:
Orsolits
B,
Vöfély
G,
Jelitai
M,
Madarász
E and
Környei
Z
(2010). Retinoid metabolism and retinoic acid signaling in astrocytes and differentiating neural stem cells.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00013
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Received:
16 Apr 2010;
Published Online:
16 Apr 2010.
*
Correspondence:
Barbara Orsolits, Hungarian Academy of Science, Institute of Experimental Medicine, Budapest, Hungary, orsolits@koki.hu