Chronic administration of apocynin and catalase ameliorates the blood pressure and renal functional parameters attributed by L-NAME induced systemic arterial hypertension in Wistar-Kyoto rats: The role of oxidative stress
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1
School of Pharmaceutical Sciences, University of Science Malaysia, Malaysia
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2
Department of Pharmaceutical Technology, School of Pharmaceutical Sciences, University of Science Malaysia, Malaysia
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3
Department of Pharmacology, School of Pharmaceutical Sciences, University of Science Malaysia, Malaysia
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4
Department of Pharmacology and Toxicology, Virginia Commonwealth University, United States
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5
Department of Physiology, University College Cork, Ireland
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6
Institute for Research in Molecular Medicine, University of Science Malaysia, Malaysia
Background
It is believed that hypertension is associated with increased vascular oxidative stress. There is still a debate whether oxidative stress is a cause or a result of hypertension. Animal studies have generally supported the hypothesis that increased blood pressure is associated with increased oxidative stress. The present study investigates the effect of apocynin-NADPH oxidase inhibitor and catalase in scavenging the free radical and oxidative stress contributed by L-NAME- a nitric oxide synthase inhibitor induced hypertension model.
Methods
Forty eight male Wistar-Kyoto rats (200-250g) were randomly assigned into 8 groups (n=6). L-NAME (15mg/kg/day p.o.) - a non-specific nitric oxide synthase inhibitor was used to induce hypertension in selected groups. Apocynin (2.5 mmol/L p.o.) and catalase (10000 U•kg-1•day-1, i.p. bolus) were administered into all the experimental models for 14 days. Weekly renal functional parameters and non-invasive blood pressure were studied. Renal hemodynamics parameters such as renal cortical blood perfusion and pulse wave velocity were also recorded. In addition to that, oxidative stress markers i.e. plasma malondialdehyde, superoxide dismutase, nitric oxide and total antioxidant capacity were used to determine the degree of oxidative stress. Kidney samples were used for histopathological studies. All the data were presented in mean±SEM and were subjected to Two-way ANOVA analysis followed by Bonferroni post hoc test with significant level at 5%.
Results
The elevation of blood pressure was observed in L-NAME treated rats on day 7 onward. Similarly, the deterioration of renal functional parameters and oxidative stress markers were also reported (all p<0.05). Renal histological studies showed that L-NAME treated rat’s experienced mild arteriolar congestion with minor inflammatory cells in cortical region. Administration of apocynin and catalase reduced blood pressure, improved renal functions and up-regulated the antioxidant defense mechanisms.
Conclusion
This study suggests that apocynin and catalase can be considered as a potential therapeutic option in future hypertension management.
Acknowledgements
The author (Tan Yong Chia) would like to thank USM Fellowship program from the Institute of Postgraduate Studies, University Sains Malaysia for all the financial supports.
References
Keywords:
Apocynin,
Catalase,
L-NAME,
Hypertension,
Oxidative Stress
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Oral Presentation
Topic:
Metabolic diseases
Citation:
Tan
Y,
Abdul Sattar
MZ,
Abdul Karim Khan
NB,
Murugaiyah
V,
Ahmed
A,
Abdullah
MH,
Ho
Y,
Kaur
G and
Johns
EJ
(2019). Chronic administration of apocynin and catalase ameliorates the blood pressure and renal functional parameters attributed by L-NAME induced systemic arterial hypertension in Wistar-Kyoto rats: The role of oxidative stress.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00066
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Received:
02 Oct 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Mr. Yong Chia Tan, School of Pharmaceutical Sciences, University of Science Malaysia, Penang, Malaysia, samual_84@hotmail.com