Evaluation of antibacterial activity and chemical constituents of Melicope glabra (Blume) T. G. Hartley
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1
Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Malaysia
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2
Department of Oral Clinical Biology, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Malaysia
Background
Melicope glabra is an edible plant which belongs to the Rutaceae family. It has been traditionally used in treating several illnesses including stomach pain, high blood pressure and to revitalize the body. In the present study, the antibacterial activity of ethyl acetate extract from the bark of M. glabra was evaluated against two oral pathogenic bacteria, Enterococcus faecalis and Streptococcus mutans. E. faecalis and S. mutans are oral pathogens that have been recognized as an important etiological agent in human dental caries. It has been suggested that E. faecalis virulence may be related to resistance to intracanal medicaments while S. mutans is a potent initiator of caries that play an important role in caries formation.
Methods
A new flavonoid compound was isolated from the ethyl acetate extract of M. glabra stem bark and its structure was elucidated by performing various spectroscopic techniques (nuclear magnetic resonance, mass spectroscopy, infrared spectroscopy) and compared with related literature data. The antimicrobial activities were evaluated by TLC-bioautography and minimum inhibitory concentration (MIC).
Results
The ethyl acetate extract exhibited good inhibition zones by TLC-bioautography method while the MIC assay showed that the extract possessed significant antibacterial activity against E. faecalis and S. mutans with the values of 5.0 mg/mL and 0.625 mg/mL respectively. Assay guided isolation and purification of ethyl acetate extract successfully isolated a new flavonoid compound; 7-hydroxy-2-(4’-hydroxy-3’-methoxy-phenyl)-3, 5-dimethoxy-chromen-4-one.
Conclusion
Ethyl acetate extract from M. glabra stem bark exhibited significant antibacterial activity and therefore can serve as a potential plant-based antimicrobial agent.
Acknowledgements
1. Department of Chemistry, Faculty of Sciences, Universiti Putra Malaysia.
2. Department of Oral Clinical Biology, Faculty of Dentistry, Universiti Kebangsaan Malaysia Kuala Lumpur.
3. Kementerian Pengajian Tinggi (Fundamental Research Grant Scheme; Vot: 5524954)
Keywords:
Melicope glabra,
Bioassay guided isolation,
Antimicrobial activity,
Enterococcus faecalis,
Streptococcus mutans
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Infectious diseases
Citation:
Mohd Zaini
H,
Kassim
N,
Zainal Abidin
Z,
Jaafar
W and
Kamal Bahrain
N
(2019). Evaluation of antibacterial activity and chemical constituents of Melicope glabra (Blume) T. G. Hartley.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00115
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Received:
27 Nov 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Nur Kartinee Kassim, Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Malaysia, kartinee@upm.edu.my