Selectively mediate proliferation and migration of human vascular endothelial cells by biodegradable carrier/gene complexes
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1
Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), China
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2
Tianjin University, School of Chemical Engineering and Technology, China
Rapid endothelialization is a prerequisite for artificial vascular vessels[1],[2]. HUVECs can be adhered specifically by REDV peptide modified artificial blood vessels, so targeting peptide modified gene carriers could promote the proliferation and migration of ECs and inhibit that of human umbilical artery smooth muscle cells (SMCs), but it is still unexplored. It is of great interest to investigate whether it is possible to develop the novel targeting biodegradable nanoparticles to condense with genes in order to realize the specific adhesion and proliferation of HUVECs. We reported a strategy for proliferation and migration of HUVECs based on targeting NPs/pDNA complexes, which exhibited outstanding endothelial cell targeting performance and low cytotoxicity. The excellent endothelial cell selective performance enabled the rapid proliferation and migration of HUVECs in the co-culture system by targeting transfection with these complexes. The targeting NP/pDNA complexes were adhered selectively by ECs, and then transfected into these cells in the co-culture system. After release from the complexes, ZNF580 gene was expressed, which is the main reason for the proliferation of ECs. Meanwhile, these NP/pDNA complexes had the targeting selective ability of ECs, namely, they could be recognized specifically and adhered efficiently by the α4β1 integrin receptor which only located on the membrane surface of ECs. Therefore, the probability of these NP/pDNA complexes entering into the SMCs reduced greatly. Targeting peptide-mediated complexes efficiently promoted the transfection and migration of endothelial cells owing to the specific selectivity of REDV to endothelial cell and the high positive charge of the carriers. The targeting NP/pDNA complexes should have great potential application for rapid endothelialization of artificial blood vessels.
National Nature Science Foundation of China (No. 31370969); Ministry of Science and Technology of China (No. 2013DFG52040)
References:
[1] Xiangkui Ren, Yakai Feng et al. Chemical Society Reviews, DOI: 10.1039/c4cs00483c
[2] Haixia Wang, Yakai Feng et al. Journal of Materials Chemistry B 2015, 3, 3379-3391
Keywords:
biomaterial,
gene delivery,
Biodegradable material,
endothelialization
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Biomaterials in gene therapy
Citation:
Feng
Y,
Li
Q,
Hao
X,
Yang
J and
Duo
X
(2016). Selectively mediate proliferation and migration of human vascular endothelial cells by biodegradable carrier/gene complexes.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.01008
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.