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Accelerated wound repair and promoted angiogenesis in a tropoelastin­ modified dermal regeneration template

Anthony Weiss1, 2, 3
  • 1 University of Sydney, Molecular Bioscience, Australia
  • 2 University of Sydney, Charles Perkins Centre, Australia
  • 3 University of Sydney, Bosch Institute, Australia

Severe burn injury results in substantial skin loss and cannot be treated by autografts. The Integra Dermal Regeneration Template (IDRT) is the leading synthetic skin substitute because it allows for wound bed regeneration and wound healing. However, all substitutes suffer from slow blood vessel ingrowth and would benefit considerably from enhanced vascularization to nurture tissue repair. It is shown here that by incorporating the human elastic protein tropoelastin into a dermal regeneration template (TDRT) we can promote angiogenesis in wound healing. In small and large animal models comprising mice and pigs, the hybrid TDRT biomaterial and IDRT show similar contraction to autografts and decrease wound contraction compared to open wounds. In mice, TDRT accelerates early stage angiogenesis by 2 weeks, as evidenced by increased angiogenesis fluorescent radiant efficiency in live animal imaging and the expression of endothelial cell adhesion marker CD146. In the pig, a full thickness wound repair model confirms increased numbers of blood vessels in the regenerating areas of the dermis closest to the hypodermis and immediately below the epidermis at 2 weeks post­surgery. It is concluded that including tropoelastin in a dermal regeneration template has the potential to promote wound repair through enhanced vascularization[1]-[3].

Australian Research Council; National Health and Medical Research Council; National Institutes of Health EB014283

References:
[1] Wang, Y., Mithieux, S.M., Kong, Y., Wang, X.Q., Chong, C., Fathi, A., Dehghani, F., Panas, E., Kemnitzer, J., Daniels, R., Kimble, R.M., Maitz, P.K., Li, Z. and Weiss, A.S. (2015) Tropoelastin incorporation into a dermal regeneration template promotes wound angiogenesis. Adv. Health. Mater. 4, 577-584.
[2] Wise, S.G., Yeo, G.C., Hiob, M.A., Rnjak-Kovacina, J., Kaplan, D.L., Ng, M.K.C. and Weiss, A.S. (2014) Tropoelastin - a versatile, bioactive assembly module. Acta Biomaterial. 10, 1532-1541
[3] Yeo, G.C., Aghaei-Ghareh-Bolagh, B., Brackenreg, E.P., Hiob, M.A., Lee, P. and Weiss, A.S. (2015) Fabricated elastin. Adv. Healthcare Mater. PMID: 25771993 in press.

Keywords: Extracellular Matrix, protein, Cell interaction, matrix-cell interaction

Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.

Presentation Type: New Frontier Oral

Topic: Biomaterials in wound care

Citation: Weiss A (2016). Accelerated wound repair and promoted angiogenesis in a tropoelastin­ modified dermal regeneration template. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01278

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Received: 27 Mar 2016; Published Online: 30 Mar 2016.