Development of epigallocatechin-gallate(EGCG) loaded collagen membrane to guide tissue regeneration
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1
State key laboratory of oral disease, China
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2
West China Hospital of Stomatology, Department of Oral implantology, China
A promising strategy to accelerate tissue regeneration is to use bio-membrane onto the surgical site.However, there is a need to control the degradation period of graft materials by related agent to assure cell behavior of wound site of healing process. But using cross-link agents also brings undesired complication because of cytotoxicity.For this reason, the idea cross-link agent with bio-active functions attracts more and more attention.In this study, we loaded EGCG on collagen membrane and investigate its ability to guide tissue regeneration by series of assays.The loaded rate and release kinetics of EGCG-loaded membrane also has confirmed and established the efficacy of EGCG as a cross-link agent. The EGCG-loaded membrane also showed excellent cytocompatibility at moderate concentrations with cells of soft and hard tissue, by cell viability, live/dead and attachment tests.Real-time PCR and ELISA were performed for investigating the changes of gene and protein expression. .Collagen membrane loaded with EGCG obviously down-regulated the expression of inflammatory-related cytokines of cells rather than collagen membrane did.Generally, our results show that EGCG-loaded membrane can be considered as a natural material with desired bio-active functions, and an effective membrane which has potency for clinical applications.
Figure 1. Cells was seeded on both with/without egcg loaded collagen membrane and performed CCK-8 assay to investigate cell viability at 1, 3 and 7 days.
Figure 2. Cells was seeded on both with/without egcg loaded collagen membrane to investigate the adhesion ability.
Keywords:
Cell Adhesion,
biomaterial,
Biocompatibility,
Tissue Regeneration
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Regenerative medicine: biomaterials for control of tissue induction
Citation:
Chu
C,
Cao
Y,
Yin
Y,
Deng
J and
Man
Y
(2016). Development of epigallocatechin-gallate(EGCG) loaded collagen membrane to guide tissue regeneration.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.02018
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.