Event Abstract

Evaluation of the growth potential and cellulase production in Cellulomonas sp. using Ulva lactuca as carbon source

  • 1 Escola Superior de Turismo e Tecnologia do Mar, Politécnico de Leiria, Portugal
  • 2 Centro de Ciências do Mar e do Ambiente (IPLeiria), Portugal

As the world hurtles toward its urban future, there is a growing need to reduce solid wastes, like organic, plastic, and paper residues as these are recurring by-products of our intensive consumer-based economic lifestyle. Poorly managed waste has a colossal impact on health, environment and economy [1,2], therefore a main concern is the recycling of waste materials using environmental friendly techniques like bioconversion. The bioconversion of organic based waste, such as paper residues, into soluble sugars (e.g. glucose) can be achieved through an enzymatically catalysed reaction [2,3] Algae represent a set of natural resources with a wide spectrum of compelling compounds, broadly used in various areas, such as food, agriculture, pharmaceuticals, cosmetics and feed. Ulva lactuca is an edible highly nutritious green algae (Chlorophyceae) rich in vitamins (e.g. thiamin, biotin, β-carotene, etc.) and fibers (57%). In addition, this specie has the advantage of having its life cycle well studied and a high production in aquaculture [4,5]. In this research, we evaluated the growth potential and cellulase production in Cellulomonas sp. using Ulva lactuca as carbon source. The growth of Cellulomonas sp., was performed for 168 hours at 30°C, with 24 hours samplings, in M9 Culture Medium (M9CM) sample tubes under different conditions: (a) [Algae]=1.16mg.mL-1; (b) [Algae]=1.16mg.mL-1 without thiamin and biotin; (c) [Algae]=2.24mg.mL-1. The results showed a better growth for samples supplemented with algae, especially in (c). The same pattern was observed in the paper degradation assay, where samples (c) showed an average concentration of soluble sugars (SS) per weight of carboard samples of 0.0124 [SS].mg-1. Assays performed with the commercial enzyme (Celluclast®) showed an average concentration of 0.427 [SS].mg-1 for similar samples. Seaweeds present promising applications as carbon sources due to their high content in fibers and proteins, while future studies could be performed exploring the potential of mainly invasive algae species.

Acknowledgements

Our special thanks to Pedro Ramalho for all the technical help provided.

References

1. Hoornweg, D., Bhada-Tata, P. (2012). What a Waste: A Global Review of Solid Waste Management. Urban development series. knowledge papers no. 15. World Bank, Washington, DC. © World Bank. https://openknowledge.worldbank.org/handle/10986/17388 License: CC BY 3.0 IGO.
2. Van Wyk, H., Mogale, A., & Moroka, S. (1999). Bioconversion of waste paper materials to sugars: An application illustrating the environmental benefit of enzymes. Biochemical Education, 27(4), 227–228. https://doi.org/10.1016/S0307-4412(99)00053-9
3. Poulsen, M. & Petersen, W. (1989). Growth and enzyme production of Cellulomonas sp. ATCC 21399 on microcrystalline cellulose. Effect of increasing concentration of a mineral medium. Applied Microbiology and Biotechnology, 30(5), 535-539. https://doi.org/10.1007/BF00263861
4. Castelló, R., Moral, A., & Ballesteros, M. (2016). Cellulose from algae as a promising alternative for papermaking. Biosaia, 4(2011), 2016. Retrieved from https://www.upo.es/revistas/index.php/biosaia/article/view/1821/1488
5. Ortiz, J., Romero, N., Robert, P., Araya, J., Lopez-Hernández, J., Bozzo, C., Rios, A. (2006). Dietary fiber, amino acid, fatty acid and tocopherol contents of the edible seaweeds Ulva lactuca and Durvillaea antarctica. Food Chemistry, 99(1), 98–104. https://doi.org/10.1016/j.foodchem.2005.07.027

Keywords: Solid waste residues, Ulva lactuca, cellulomonas sp., Cellulase, Cellulose, Bioconversion of paper waste

Conference: IMMR'18 | International Meeting on Marine Research 2018, Peniche, Portugal, 5 Jul - 6 Jul, 2018.

Presentation Type: Poster Presentation

Topic: Blue Biotech

Citation: Alves J, Teodoro F and Cruz A (2019). Evaluation of the growth potential and cellulase production in Cellulomonas sp. using Ulva lactuca as carbon source. Front. Mar. Sci. Conference Abstract: IMMR'18 | International Meeting on Marine Research 2018. doi: 10.3389/conf.FMARS.2018.06.00155

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Received: 06 May 2018; Published Online: 07 Jan 2019.

* Correspondence:
Miss. Joana Alves, Escola Superior de Turismo e Tecnologia do Mar, Politécnico de Leiria, Peniche, Portugal, joana.abr.alves@gmail.com
Mr. Fernando Teodoro, Escola Superior de Turismo e Tecnologia do Mar, Politécnico de Leiria, Peniche, Portugal, fjtdgm@gmail.com

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