An investigation of the effects of Cinnamomum cassia bark extracts on oxidative DNA damage and possible cytotoxic and apoptotic activities in transformed/untransformed cell lines from Type 1 diabetic patients, in vitro.
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1
Ege University, Faculty of Pharmacy, Pharmaceutical Toxicology, Türkiye
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2
Ege University, Faculty of Medicine, Dept of Medical Biology, Türkiye
It was shown that patients with Type 1 diabetes mellitus (T1DM) had increased level of oxidative DNA damage and decreased efficacy of DNA repair. These changes were implicated in the increased cancer risk in patients with diabetes mellitus. Cinnamon bark extracts have diverse biological activities including antidiabetic and anti-tumor properties. Cinnamomum cassia (C. cassia) is a common used cinnamon species present in commercial cinnamon preparations. We aimed to investigate the effects of cinnamon extracts prepared from C. cassia bark on endogenous and hydrogen peroxide (H2O2)-induced oxidative DNA damage, as well as cytotoxic and apoptotic activities in this study. Type 1 diabetic (T1DM) lymphocytes (GM02765, GM01838) and fibroblasts (GM01837) were obtained from NIGMS Human Genetic Cell Repository of Coriell Institute, New Jersey, USA. Cytotoxicity analysis were performed by using a tetrazolium salt, 4-[3-(4-iodophenyl) 2-(4-nitrophenyl) 2H-5-tetrazolio] 1,3-benzene disulfonate (WST-1). The effects of extracts on endogenous and H2O2-induced oxidative DNA damage were studied using the single cell gel electrophoresis (SCGE; Comet Assay), a technique allowing DNA damage in a single cell. Apoptotic activities of extracts were investigated by TUNEL and Annexin V/PI assays. using flow cytometry. IC50 and IC20 values of the extracts varied and the effects on endogenous and H2O2-induced DNA damage were different regarding cell lines and extracts. Although their protective effects at some doses against to H2O2-induced oxidative damage, our results suggested DNA damaging and apoptotic potential of cinnamon bark extracts on Type 1 diabetic cell lines, in vitro.
Acknowledgements
This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK ; Project number is 112S120). We also thank Prof. Dr. Erdal Bedir from Department of Bioengineering, Ege University, for identifying the Cinnamomum cassia bark and also for his advisory for preparation of cinnamon bark extracts.
References
Zhang, G., Gurtu, V., Kain, S.R., Yan, G. (1997). Early Detection of Apoptosis Using a Fluorescent Conjugate of Annexin V. Biotechniques 23, 525–531.
Kim, S.Y., Lee, C.H., Ahn, Y.J. (2004). Cytotoxic and Mutagenic Effects of Cinnamomum Cassia Bark-Derived Materials. J Microbiol Biotechnol 14(6), 1176–1181.
Koppikar, S.J., Choudhari, A.S., Suryavanshi, S.A., Kumari, S., Chattopadhyay, S., Kaul-Ghanekar, R. (2010). Aqueous Cinnamon Extract (ACE-c) from the bark of Cinnamomum cassia causes apoptosis in human cervical cancer cell line (SiHa) through loss of mitochondrial membrane potential. BMC Cancer 10, 210.doi:10.1186/1471-2407-10-210
Singh, N.P., McCoy, M.T., Tice, R.R., Schneider E.L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175, 184-191.
Keywords:
Cinnamomum cassia,
oxidative DNA damage,
Apoptosis,
Flow Cytometry,
Comet Assay,
type 1 diabetes mellitus
Conference:
ICAW 2015 - 11th International Comet Assay Workshop, Antwerpen, Belgium, 1 Sep - 4 Sep, 2015.
Presentation Type:
Poster Presentation
Topic:
Clinical applications of the comet assay
Citation:
Lermioglu Erciyas
F,
Agrap
B,
Çanaklı
G,
Celiksoz
M,
Sozer
S and
Gunduz
C
(2015). An investigation of the effects of Cinnamomum cassia bark extracts on oxidative DNA damage and possible cytotoxic and apoptotic activities in transformed/untransformed cell lines from Type 1 diabetic patients, in vitro..
Front. Genet.
Conference Abstract:
ICAW 2015 - 11th International Comet Assay Workshop.
doi: 10.3389/conf.fgene.2015.01.00006
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Received:
02 Jun 2015;
Published Online:
23 Jun 2015.
*
Correspondence:
Prof. Ferzan Lermioglu Erciyas, Ege University, Faculty of Pharmacy, Pharmaceutical Toxicology, BORNOVA-IZMIR, 35100, Türkiye, ferzanl90@gmail.com