Genotoxic effects of bismuth (III) oxide nanoparticles by comet assay
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1
Uşak University, Molecular Biology and Genetics, Türkiye
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2
Üsküdar University, Molecular Biology and Genetics, Türkiye
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3
Afyon Kocatepe University, Molecular Biology and Genetics, Türkiye
Bismuth oxide is one of the important transition metal oxides and it has been intensively studied due to their peculiar characteristics (semiconductor band gap, high refractive index, high dielectric permittivity, high oxygen conductivity, resistivity, photoconductivity and photoluminescence etc.). Therefore, it is used such as microelectronics, sensor technology, optical coatings, transparent ceramic glass manufacturing, nanoenergetic gas generator, biosensor for DNA hybridization, potential immobilizing platforms for glucose oxidase and polyphenol oxidase, fuel cells, a additive in paints, an astringent in a variety of medical creams and topical ointments, and for the determination of heavy metal ions in drinking water, mineral water and urine. In addition this, Bismuth (III) oxide nanoparticles (BONPs) are favorable for the biomolecules adsorption than regular sized particles because of their greater advantages and novel characteristics (much higher specific surface, greater surface free energy, and good electrochemical stability etc.).
Genotoxic effects of BONPs were investigated on the root cells of Allium cepa by Comet assay. A. cepa roots were treated with the aqueous dispersions of BONPs at 5 different concentrations (12.5, 25, 50, 75, and 100 ppm) for 4 h. A significant increase in DNA damage was also observed at all concentrations of BONPs except 12.5 ppm by Comet assay. The results were also analyzed statistically by using SPSS for Windows; Duncan’s multiple range test was performed. These result indicate that BONPs exhibit genotoxic activity in A. cepa root meristematic cells.
Keywords:
Allium,
Comet,
DNA Damage,
bismuth oxide,
Nanoparticles
Conference:
ICAW 2015 - 11th International Comet Assay Workshop, Antwerpen, Belgium, 1 Sep - 4 Sep, 2015.
Presentation Type:
Poster Presentation
Topic:
Genotoxicity testing of chemicals and nanomaterials
Citation:
Liman
R,
Konuk
M and
Ciğerci
İH
(2015). Genotoxic effects of bismuth (III) oxide nanoparticles by comet assay.
Front. Genet.
Conference Abstract:
ICAW 2015 - 11th International Comet Assay Workshop.
doi: 10.3389/conf.fgene.2015.01.00010
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Received:
13 May 2015;
Published Online:
23 Jun 2015.
*
Correspondence:
Dr. Reecep Liman, Uşak University, Molecular Biology and Genetics, Uşak, Türkiye, recep.liman@usak.edu.tr