Testing the genotoxicity of some perfumes with high diethylphthalate (DEP) levels using comet assay
-
1
King Faisal Specialist Hospital & Research Centre, Saudi Arabia
The presence of phthalates in perfumes has gained recently some attention since these chemicals are added sometimes intentionally as a fixative. Our previous study tested 47 branded perfumes sold in Saudi market and found 68% of tested samples had diethylphthalate (DEP), above reported threshold limit of 1 ppm. Of these phthalates, DEP was found to have the highest mean value (1621.63 ppm). These results enticed us to test the potential genotoxicity of 7 brands in which their DEP contents were in the range of 1.06 ppm to 23649.3 ppm in human TK-6 cells using single cell gel electrophoresis assay (comet assay). Cells were exposed to 400 µl perfume for 2 hrs, at room temperature. Tail moment was (TM) used as a metric measure for DNA damage and 25 cells per sample were determined by image analysis software. Four perfumes with DEP above 40 ppm produced significant DNA damage in TK-6 cells with TM of 54.27 ± 1.04, n=500 compare to the other 3 perfumes with DEP < 2 ppm (21.94 ± 1.09, n=300), untreated cells (2.99 ± 0.17, n=250) and cells induced with ethanol (33.76 ± 1.4, n=75) or methanol (23.82 ± 1.84, n=100 which are the vehicles used in perfume's preparations. The results suggest that DEP in perfumes might be one of the ingredients that provoked DNA damage; however, further investigation is required confirming our observation.
Keywords:
Perfumes,
phthalates,
Comet Assay,
Tail moment,
Diethylphthalate
Conference:
ICAW 2015 - 11th International Comet Assay Workshop, Antwerpen, Belgium, 1 Sep - 4 Sep, 2015.
Presentation Type:
Poster Presentation
Topic:
Genotoxicity testing of chemicals and nanomaterials
Citation:
Al-Saleh
I,
Al-Rajudi
T and
Alqudaihi
G
(2015). Testing the genotoxicity of some perfumes with high diethylphthalate (DEP) levels using comet assay.
Front. Genet.
Conference Abstract:
ICAW 2015 - 11th International Comet Assay Workshop.
doi: 10.3389/conf.fgene.2015.01.00039
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
04 May 2015;
Published Online:
23 Jun 2015.
*
Correspondence:
Dr. Iman Al-Saleh, King Faisal Specialist Hospital & Research Centre, Riyadh, Saudi Arabia, iman@kfshrc.edu.sa