Ecogenotoxicity testing of aquatic environment by comet assay in plants
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1
University of Calcutta, Department of Botany, India
One of the goals of environmental monitoring is the detection of potentially hazardous compounds in water. We have set up a standard method to apply the Comet assay in aquatic plants that could be of great interest to evaluate cytotoxicity, genotoxicity and oxidative stress on the same species regarded as most sensitive to environmental pollutants.
The aim of the present study was to set up of standardized procedure to evaluate genotoxicity in aquatic plants- Ceratophyllum demersum one that is submerged free floating and the other is Lemna minor - a fresh water floating plant by Comet assay. Electrophoresis and unwinding times were adapted to obtain minimum DNA migration evaluated as tail intensity % or tail moment in the control group and, at the same time maximum sensitivity for DNA damage with known genotoxicants. We further investigated the cytotoxicity and oxidative stress induced in the same species. Based on the repeatability of results obtained we suggest that Ceratophyllum, Lemna can serve as model species and Comet assay could be adopted to monitor the eco-genotoxicity of water pollutants.
Acknowledgements
University Grants Commission-RFSMS [Sanction No. 5-21/2007(BSR) dt. 13/12/2012]
University Grants Commission-Major Project-41-1116/2012(SR) dt. 26.7.2012
Keywords:
Ceratophyllum,
Lemna,
Comet Assay,
Fly Ash Leachate,
Antioxidant Enzymes
Conference:
ICAW 2015 - 11th International Comet Assay Workshop, Antwerpen, Belgium, 1 Sep - 4 Sep, 2015.
Presentation Type:
Oral Presentation
Topic:
Ecogenotoxicology
Citation:
Mukherjee
A,
Jana
A,
De
A,
Ghosh
I and
Chakrabarti
M
(2015). Ecogenotoxicity testing of aquatic environment by comet assay in plants.
Front. Genet.
Conference Abstract:
ICAW 2015 - 11th International Comet Assay Workshop.
doi: 10.3389/conf.fgene.2015.01.00051
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Received:
15 May 2015;
Published Online:
23 Jun 2015.
*
Correspondence:
Prof. Anita Mukherjee, University of Calcutta, Department of Botany, Kolkata, 700019, India, anitamukherjee28@gmail.com