Comet and DNA repair capacity in newborns
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1
Laboratory of Cell Genetics, Faculty of Science and Bio-engineering, Vrije Universiteit Brussel, Belgium
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2
Belgian Cancer Registry, Belgium
Despite many efforts to improve human health, the incidence in childhood cancer is increasing. Since relevant data regarding biomonitoring of newborns to unintended mutagen exposure are increasing too slow, the susceptibility of newborns (preterm and full term) to mutagens was assessed by comparison with their mothers using the comet assay in well characterized small Belgian study population of mother-newborn pairs. In a first part, background DNA damage, in vitro H2O2 induced oxidative DNA damage and repair capacity (residual DNA damage) in peripheral blood mononucleated cells (PBMCs) from 25 preterm newborns and their mothers were assessed using a cellular repair assay previously developed in our laboratory. In addition, demographic data were taken into account and the repair capacity of preterm was compared to full term newborns. Next a NER repair phenotype assay was used to assess the repair capacity of 25 full term newborns and their mothers, taking into account demographic data. This to investigate how well newborns are able to cope with “manmade” induced DNA damage.
Keywords:
Comet Assay,
biomonitoring,
newborns,
Birth cohort,
DNA Repair
Conference:
ICAW 2015 - 11th International Comet Assay Workshop, Antwerpen, Belgium, 1 Sep - 4 Sep, 2015.
Presentation Type:
Oral Presentation
Topic:
BEMS satellite meeting
Citation:
Kirsch-Volders
M,
Decordier
I and
Vande.Loock
K
(2015). Comet and DNA repair capacity in newborns.
Front. Genet.
Conference Abstract:
ICAW 2015 - 11th International Comet Assay Workshop.
doi: 10.3389/conf.fgene.2015.01.00074
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Received:
24 Aug 2015;
Published Online:
24 Aug 2015.
*
Correspondence:
Dr. Kim Vande.Loock, Laboratory of Cell Genetics, Faculty of Science and Bio-engineering, Vrije Universiteit Brussel, Brussels, Belgium, Kim.Vande.Loock@vub.ac.be