House dust mite-sensitized myeloid dendritic cells induce reduction of allergen-specific murine airway inflammation
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1
Research Center Gonçalo Moniz- Oswaldo Cruz Foundation, Brazil
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2
Universidade Federal da Bahia, Brazil
Allergies affect about 20 to 30% of the world population and its prevalence, as well as the severity of their symptoms, has been increasing in recent decades. Blomia tropicalis (Bt) has been implicated as one of the most important causative agents of allergic diseases in many latin-american countries, been related to asthma, atopic dermatitis and rhinitis. Dendritic cells (DCs) in a state of partial maturation have a tolerogenic profile, being able to induce immunological tolerance. In this work, the effect of intraperitoneally injected myeloid DCs that had been in vitro sensitized with a Bt extract (BtE) was evaluated in an experimental respiratory allergy model. In allergic models, allergen-specific T helper type 2 (Th2) cells produce key cytokines that regulate the synthesis of allergen-specific IgE and control tissue eosinophilic airway inflammation and airway remodeling. These parameters were evaluated in mice that were intraperitoneally immunized with aluminium hydroxide-adsorbed BtE and intranasally challenged with soluble BtE. The previous inoculation of BtE-sensitized DCs significantly reduced the total number of leukocytes and the number of eosinophils in bronchoalveolar lavage fluid and reduced the production of IgE serum. In addition, reduced lung inflammatory infiltrate was observed in animals that had been treated either with BtE-sensitized DCs or with ovalbumin-sensitized DCs. Those results indicate that the use of tolerogenic DCs should be further investigated as a possible therapeutic strategy for the control of allergen-triggered airway inflammation.
Keywords:
tolerance,
mite,
dendritic cell,
airway inflammation,
Blomia tropicalis
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Translational immunology and immune intervention
Citation:
França
LA,
Costa
FB,
Athanazio
D,
Neves
NA and
Carvalho
LP
(2013). House dust mite-sensitized myeloid dendritic cells induce reduction of allergen-specific murine airway inflammation.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00012
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Received:
08 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Lain P Carvalho, Research Center Gonçalo Moniz- Oswaldo Cruz Foundation, Salvador, Brazil, lain.carvalho@gmail.com