The peripheral blood compartment of patients with Diffuse Large B Cell Lymphoma (DLBCL) at diagnosis is characterized by distinct phenotypic and functional Immunological alterations
Simone
Battella1*,
Sabrina
Pelliccia2,
Raffaella
La Scaleia1,
Arianna
Di Napoli3, 4,
Alessandra
Porzia1,
Luigi
Ruco3, 4,
Fabrizio
Mainiero1,
Angela
Santoni5,
Christina
Cox2 and
Gabriella
Palmieri1
-
1
La Sapienza University, Department of Experimental Medicine, Italy
-
2
S'Andrea Hospital, Hematology unit, Italy
-
3
La Sapienza University, Department of Clinical and Molecular Medicine, Italy
-
4
S'Andrea Hospital, Pathology Unit, Italy
-
5
La Sapienza University, Deparment of Molecular Medicine, Italy
Tumor ability to modify the immune competence of the host may be particularly relevant in the case of hematological malignancies, such as B-cell lymphoma. This phenomenon might hamper the efficacy of chemo-immunotherapy.
We analyzed the phenotypic and functional asset of PBMC in 30 DLBCL at diagnosis (as compared with 23 healthy, age- and sex-matched controls), and the possible correlation of the immunological profile with pathological and clinical traits.
Patients showed a higher absolute monocyte number, and a lower lymphocyte count, mostly due to the selective diminution of CD4+ T cells (but not of Treg cells) and B lymphocytes. Accordingly, lymphocyte/monocyte and CD4/CD8 ratios decreased, while NK percentage increased.
The phenotypically skewed profile of PBL was associated with functional alterations: 1) IFN-γ producing cells were increased among CD4+ and CD8+ T cell subsets, but not in CD56+ T and NK cell populations; 2) a higher frequency of Granzyme B-expressing cells characterized T and NK subsets; noteworthy, natural and CD16-dependent NK cytotoxic activities were unchanged. IL-6 and IL-10 plasma levels were significantly higher in DLBCL.
Selected alterations differently correlated with the DLBCL Germinal Genter B (GCB) vs non GCB-type and with the presence of bulky disease or extranodal involvement. Our findings show a deeply altered phenotypic and functional profile in DLBCL PBMC populations, with the lymphocyte
compartment showing traits of chronic activation; moreover, distinct immunological alterations correlated with histological subtypes and other clinical features. SB and RLS equally contributed to the study; MCC and GP equally contributed to the study
Keywords:
Immune profiling,
T cell effector functions,
B cell lymphoma,
IFN gamma,
NK cells
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Battella
S,
Pelliccia
S,
La Scaleia
R,
Di Napoli
A,
Porzia
A,
Ruco
L,
Mainiero
F,
Santoni
A,
Cox
C and
Palmieri
G
(2013). The peripheral blood compartment of patients with Diffuse Large B Cell Lymphoma (DLBCL) at diagnosis is characterized by distinct phenotypic and functional Immunological alterations.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00034
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Received:
08 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Simone Battella, La Sapienza University, Department of Experimental Medicine, Rome, Italy, simone.battella@uniroma1.it